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Identification method of resistance to live Spodoptera litura larvae in soybean seedling stage

A technology of Spodoptera litura and identification method, which is applied in botany equipment and methods, leguminous plant cultivation, application, etc., can solve the problems of large error, difficult identification, heavy workload, etc., achieve low cost and improve larval survival High efficiency and practical effect

Active Publication Date: 2020-01-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The identification of insect resistance involves two kinds of organisms, plants and pests, which is difficult to identify and requires a lot of work. At the same time, the error is often large

Method used

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  • Identification method of resistance to live Spodoptera litura larvae in soybean seedling stage
  • Identification method of resistance to live Spodoptera litura larvae in soybean seedling stage
  • Identification method of resistance to live Spodoptera litura larvae in soybean seedling stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Resistance identification of soybean insect-resistant core germplasm to Spodoptera litura larvae at seedling stage

[0043] Step 1: Selection and production of live selection resistance identification sites at the seedling stage:

[0044] A greenhouse with good ventilation, good light and high terrain was selected in Nanjing Agricultural University, and a self-made closed net room was made in the greenhouse to identify the resistance of soybean seedlings to Spodoptera litura larvae. Such as figure 1 As shown, the self-made closed net room structure includes: 4 stainless steel pipes with a length of 150 cm, one end is cut into a sharp wedge shape to facilitate insertion into the soil and fixed, as a pillar supporting the net room; a self-made 100-mesh cuboid with zippers on both sides is sealed The net room, cloth strips are sewn above the net room to fix and support the net room; the bamboo poles supporting the net room are cut according to the size of the n...

Embodiment 2

[0062] Embodiment 2: Comparison of 32 holes and 50 holes seedling trays

[0063] Using 5 insect-resistant varieties and 4 insect-susceptible varieties, a total of 9 varieties, randomized block design, 4 repetitions, comparative analysis of the size of the seedling trays was carried out.

[0064] Compared with the 50-hole seedling tray, the 32-hole seedling tray has more water, space and nutrient soil supply in each hole, which can ensure the neat emergence of each tray, and the soybean plants grow relatively robustly in the 32-hole seedling tray.

[0065] Compared with the 50-hole seedling tray, the 32-hole seedling tray can easily and accurately investigate the leaf area loss value of different varieties because the distance between each hole is relatively large.

[0066] Such as Figure 7 As shown, during the whole survey period of the experiment, the F values ​​of 32-hole seedling trays were higher than those of 50-hole seedling trays on most survey days, indicating that t...

Embodiment 3

[0069] Embodiment 3: the selection resistance evaluation of large sample recombinant inbred line population (containing 326 families)

[0070] This method was used to evaluate the seedling resistance of recombinant inbred line NJRILY to living larvae of Spodoptera litura. The population was derived from the crossing of the highly resistant parent Lamar and the susceptible parent Nannong 1138-2, including 326 families. Because there are many families in this group, one net room can only do one repetition, that is, one block. Such as Figure 9 It was shown that when the average leaf area loss value of three replicates of the NJRILY population reached 50%, the leaf area loss value of the family was normally distributed and there was super-parent segregation. Analysis of variance found that there were extremely significant differences in leaf area loss values ​​among families, indicating that this method can be used for phenotypic identification of large populations, and the res...

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Abstract

The invention discloses an identification method for selection resistance of prodenia litura larva living bodies at the soybean seedling stage and belongs to the technical field of plant insect resistance identification. The method includes the following steps that 1, a seedling stage living body selection resistance identification place is selected and manufactured; 2, a selection resistance identification soybean material and an anti-fever standard variety are planted in a 32-hole nursery site; 3, prodenia litura insect sources are artificially propagated; 4, uniform insect inoculation is carried out artificially at the seedling stage; 5, the insect resistance is investigated and identified. According to the method, interference of non-biological and biological factors is ingeniously reduced through combination of a greenhouse and a netting house, the testing scale is reduced by planting the soybean material in the nursery site, the testing period is shortened through uniform insect inoculation identification at the seedling stage, therefore, the selection resistance of the soybean material can be objectively, accurately and rapidly evaluated, high-resistance and high-sensitivity materials are screened out, and the identification period is greatly shortened. Meanwhile, the method is simple, easy to carry out, low in cost, high in operability and very prominent in practicality, the method can efficiently and accurately carry out prodenia litura larva selection resistance evaluation and insect-resistant material screening on soybeans on a large scale.

Description

technical field [0001] The invention belongs to the technical field of identification of plant insect resistance, and in particular relates to an identification method of soybean seedling resistance to live Spodoptera litura larvae. Background technique [0002] Spodoptera litura Fabricius belongs to Lepidoptera, Noctuidae, has another name called Spodoptera litura. It is a kind of leaf-eating pest with strong gluttony and omnivory. The endangered plants include dicotyledonous and monocotyledonous plants. A total of 109 families and 389 species. Spodoptera litura can eat the leaves, flower buds, flowers and fruits of plants. The newly hatched larvae eat the lower epidermis and mesophyll of the leaves. After being harmed, the epidermis of the leaves will become transparent spots; Lower main vein. Spodoptera litura is the main pest on soybean in southern my country. [0003] Integrated pest management mainly includes chemical, physical and biological control methods, among ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/40A01K67/033
CPCA01G22/00A01K67/033
Inventor 邢光南刘凯盖钧镒
Owner NANJING AGRICULTURAL UNIVERSITY
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