Method for establishing model of aflatoxin inducing macaque liver fibrosis

A technology for aflatoxin and liver fibrosis, which is used in pharmaceutical formulations, compound screening/testing, preparations for in vivo tests, etc. Mold efficiency, improve the success rate of modeling, reduce the effect of modeling time

Inactive Publication Date: 2017-02-22
YUNNAN KEY LAB OF PRIMATE BIOMEDICINE RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no direct evidence and understanding of the carcinogenic effect of aflatoxin and the etiology, course and pathology of liver fibrosis

Method used

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  • Method for establishing model of aflatoxin inducing macaque liver fibrosis
  • Method for establishing model of aflatoxin inducing macaque liver fibrosis
  • Method for establishing model of aflatoxin inducing macaque liver fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Randomly select 2 males and 1 female from healthy rhesus monkeys, aged 12 years, 1 male weighing 8.1Kg and 7.9Kg each, and 1 female weighing 5.3Kg as model animals. During the modeling period, 0.5 mg / kg of aflatoxin (aflatoxin B1 manufacturer: sigma product number: A6636 specification: 50 mg powder) was directly added to the drinking water of the macaques for sucking, twice a month , each interval of 2 weeks, 8 consecutive months, 8 consecutive months, and 2 times a month, each interval of 2 weeks, serological index detection at the end of each month, the first month and 3 months after modeling, Serum results at 6 months and 8 months are as follows figure 1 . Through pathological examination, macaques showed obvious symptoms of liver fibrosis. At the same time, 2 males and 1 female were randomly selected from healthy rhesus monkeys as controls, aged 12 years, weighing 5-8 Kg, and fed DMSO according to their body weight. During the modeling period, the nutritional com...

Embodiment 2

[0030] The drinking water preparation method of Example 1 is: weigh 10 mg of aflatoxin, repeatedly blow it to make it fully dissolved in 1 mL of DMSO, preheat normal saline at 50°C to 60°C in advance, and dilute the mixture containing aflatoxin with normal saline DMSO to 100mL, according to 0.5mg / kg body weight of the animal, it was divided into special aseptic drinking bottles for macaques to suck. All the other are consistent with embodiment 1.

[0031] (2) Evaluation methods and results of model building

[0032] Evaluation methods: The diagnostic model of liver fibrosis mainly includes comprehensive diagnosis of serum biochemical markers, imaging, and histopathology.

[0033] 1. Serum biochemical markers include hyaluronic acid (HA), laminin (LN), type III procollagen V-telopeptide (PIIINP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total protein (TP) Wait. Serological index test results.

[0034] During the modeling period, the serum of the...

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Abstract

The invention relates to a method for establishing a model of aflatoxin inducing macaque liver fibrosis. The method comprises the following steps: with macaque as a model animal, during the model making, adding 0.5mg/kg aflatoxin to the drinking water of macaque for sucking, twice every month at an interval of 2 weeks for 8 successive months; feeding the macaque with macaque commercial maintenance-period feed containing more than 19% of protein and more than 4% of crude fat; performing pathological analysis every 2 months; and 8 months later, performing pathological detection to show obvious liver fibrosis symptoms. The method provided by the invention is safe and reliable, shortens the model making time of an animal model, and is of great significance to screening and predicting effective drugs for controlling liver fibrosis and even liver cancer as an experimental foundation.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, and in particular relates to a method for establishing a liver fibrosis model. Background technique [0002] Hepatic fibrosis refers to intrahepatic fibrous hyperplasia under the action of various etiologies, which may be accompanied by the formation of fibrous septa. Liver fibrosis is mainly a pathological concept, which refers to the pathological changes of abnormal changes in liver structure and / or function caused by excessive proliferation and abnormal deposition of extracellular matrix components in liver tissue. The pathological mechanism is mostly due to the necrosis of hepatic parenchymal cells, leading to the collapse of the liver mesh scaffold, which is a passive and irreversible pathological process. The construction of experimental animal models similar to the etiology, course and pathology of human liver fibrosis is the basis for screening and predicting effective drugs t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61K49/00
CPCA61K31/343A61K49/0008
Inventor 司维王宏严亚萍王峻峰常绍辉段艳超牛昱宇季维智
Owner YUNNAN KEY LAB OF PRIMATE BIOMEDICINE RES
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