Method for constructing rat model with pine cone injury
A rat model and construction method technology, applied in animal husbandry and other directions, can solve the problems of complex operation, poor repeatability, and long modeling time of pineal injury model, achieving strong repeatability, short modeling time, Simple modeling method
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[0029] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the rest of the present disclosure in any way.
[0030] 1. Materials:
[0031]1. Animal: rat, SD species, clean grade, male, weighing 160-180 g, purchased from Beijing Huafukang Biotechnology Co., Ltd., license number: SCXK (Beijing) 2014-0004, certificate number: No .1103221911001384. Animal breeding conditions: Breed in the central laboratory of Shanxi Academy of Traditional Chinese Medicine, temperature: 20°C-23°C, humidity: 20%-50%.
[0032] 2. Reagents: p-chlorophenylalanine (PCPA), Sigma, USA, batch number: 1002600696; sodium pentobarbital, Sigma, USA, batch number: 57-33-0; anhydrous sodium carbonate, Beijing Chemical Plant, batch number Sodium bicarbonate, Beijing Chemical Plant, batch number: 2...
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