Method for constructing rat model with pine cone injury

A rat model and construction method technology, applied in animal husbandry and other directions, can solve the problems of complex operation, poor repeatability, and long modeling time of pineal injury model, achieving strong repeatability, short modeling time, Simple modeling method

CN111642460AActive Publication Date: 2020-09-11SHANXI PROVINCE CHINESE MEDICINE RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2020-09-11

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Abstract

The invention belongs to the technical field of experimental animal models, and provides a method for constructing a rat model with pine cone injury, which aims to solve the problems of complex operation, low success rate, long modeling time, low repeatability and the like of the conventional rat model with pine cone injury. The method comprises the following steps of: performing intraperitoneal injection of PCPA of 450 mg / kg on a rat for 2 days continuously, once every day; or performing electrical stimulation on the rat by using a shuttle box, wherein the electrical stimulation voltage is 30V, the current is 0.8A, the stimulation time is 30 seconds, the interval is 30 seconds, the cycle is performed for 60 times, and the operation is performed for 5 days continuously; or performing an intraperitoneal injection PCPA method on the rat on the fourth day by using an electrical stimulation method. Three modeling methods, namely pathological histomorphology, serum detection of the contentof melatonin which is a main synthetic secretory hormone of a pine cone, and animal general situation and behavioral auxiliary method objective evaluation, cause injury of the pine cone of the rat, and provide a good model basis for researching and screening medicaments and mechanisms with protective and therapeutic effects on the pine cone injury; and the method is simple and convenient, the modeling time is short, the repeatability is high, and the index evaluation is simple and reliable.
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Description

technical field

[0001] The invention belongs to the technical field of experimental animal models, and in particular relates to a method for constructing a pineal gland injury rat model. Background technique

[0002] The pineal gland (pineal gland) is an important part of the body's biological clock rhythm [1], and is the core component that maintains the operation of the endogenous biological clock. It synthesizes and secretes melatonin (MT), which has the functions of promoting sleep, regulating jet lag, Anti-aging, immune regulation, anti-tumor and other functions, because it can promote sleep, it is called "physiological hypnotic agent" [2]. As the pineal gland grows older, the pineal gland cells become larger, the vacuoles change, the synthesis and secretion of melatonin decreases, and its role in regulating the circadian rhythm decreases. At present, the model of pineal gland injury has been established by separating the left common carotid artery and permanently liga...

Examples

Embodiment Construction

[0029] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the rest of the present disclosure in any way.

[0030] 1. Materials:

[0031]1. Animal: rat, SD species, clean grade, male, weighing 160-180 g, purchased from Beijing Huafukang Biotechnology Co., Ltd., license number: SCXK (Beijing) 2014-0004, certificate number: No .1103221911001384. Animal breeding conditions: Breed in the central laboratory of Shanxi Academy of Traditional Chinese Medicine, temperature: 20°C-23°C, humidity: 20%-50%.

[0032] 2. Reagents: p-chlorophenylalanine (PCPA), Sigma, USA, batch number: 1002600696; sodium pentobarbital, Sigma, USA, batch number: 57-33-0; anhydrous sodium carbonate, Beijing Chemical Plant, batch number Sodium bicarbonate, Beijing Chemical Plant, batch number: 2...