Building method of rotavirus infection suckling mouse experiment model

A rotavirus and experimental model technology, applied in the field of experimental zoology, can solve the problems of high breeding conditions, low modeling success rate, and expensive experimental animals, achieving high modeling success, short modeling time, The effect of saving experimental cost

Inactive Publication Date: 2017-02-22
湖北华冠中科生物药业有限公司 +3
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  • Abstract
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Problems solved by technology

[0003] At present, regarding the construction of rotavirus animal models, there are sterile piglets, rhesus monkeys and rabbits as exp...

Method used

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  • Building method of rotavirus infection suckling mouse experiment model
  • Building method of rotavirus infection suckling mouse experiment model
  • Building method of rotavirus infection suckling mouse experiment model

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with the examples, but this description will not constitute a limitation to the present invention. Example

[0022] (1) Rotavirus culture

[0023] 1. Cell culture:

[0024] MA104 cells were grown and passaged in DMEM medium containing 10% fetal bovine serum, 100 μ / mL penicillin, and 100 μg / mL streptomycin.

[0025] 2. Virus amplification:

[0026] MA104 cells were inoculated with RV when grown to a monolayer in culture flasks. The original virus liquid, ie non-sialic acid (SA) dependent human rotavirus WA strain (commercially available), was taken out from the -80°C refrigerator and dissolved at 4°C. The MA104 cells grown into a monolayer were washed twice with PBS. Then add the virus liquid, put it into a 37° C. carbon dioxide incubator (containing 5% carbon dioxide) and incubate for 2 hours, discard the virus liquid, and add DMEM culture liquid without fetal bovine serum. Then put it ...

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Abstract

The invention relates to a building method of a rotavirus infection suckling mouse experiment model. The method concretely comprises the following steps that 7-day-old ICR suckling mice are used as experimental animals; a 1ml improved injector is used; 0.2ml of rotavirus liquid is given in an intragastric administration mode. The model provided by the invention can simulate features of infant rotavirus infection acute gastroenteritis; the rotavirus infection suckling mouse experiment model is fast built; the sufficient number of mice infecting the rotavirus are obtained; the stability is good; the repeatability is realized; the model building success rate is high and is nearly 100 percent. In addition, the technical scheme provided by the invention has the advantages that the weight of the selected low-age animals is light; the obtaining is easy; the used virus dose is small; the experiment cost is reduced; the method is applicable to batch modeling.

Description

technical field [0001] The invention belongs to the field of experimental zoology, and in particular relates to a method for constructing an experimental model of suckling mice infected with rotavirus. Background technique [0002] Rotavirus is an important pathogen that causes acute diarrhea and death in infants under 5 years old, accounting for more than 50% of all intestinal infections. Every year, about 200 million infants around the world are infected with RV and develop diarrhea, and about 1 million children die from the disease. Currently there is no effective treatment. For this reason, more and more scholars at home and abroad have paid more and more attention to the establishment of animal models of rotavirus infection to study the pathogenic mechanism of rotavirus and the efficacy of related vaccines and drugs. [0003] At present, regarding the establishment of animal models of rotavirus, there are sterile piglets, rhesus monkeys and rabbits as experimental ani...

Claims

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Application Information

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IPC IPC(8): A61K35/76
CPCA61K35/765
Inventor 李华蔡勇黄红兰梅亚红邹昌勇席甘何卫华杨荣阁孙宾莲
Owner 湖北华冠中科生物药业有限公司
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