ITS (internal transcribed spacer) sequence of dalbergia odorifera and method for identifying dalbergia odorifera by ITS sequence

An identification method of Dalbergia balsamicus, applied in the field of identification of plant species, can solve the problems of difficult identification of mahogany, high requirements for equipment hardware, and high limitations, and is conducive to large-scale promotion and use, with high identification accuracy, Simple operation effect

Inactive Publication Date: 2017-02-22
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional mahogany identification and distinction are mainly based on macroscopic characteristics (such as density, structure, material color and texture, etc.), supplemented by necessary wood microanatomical methods to determine its genus and species. Wood morphology and anatomy knowledge and identification experience, and because of the similar morphological characteristics between closely related species, traditional morphological and anatomical identification methods are usually difficult to accurately identify wood to the level of species, which brings great difficulties to the identification of mahogany , unable to meet market demand
Shen Mingyue et al. (2012) used high-performance liquid chromatography (HPLC), Zhu Tao et al. (2013) used gas chromatography-mass spectrometry (GC-MS) to successfully identify species of redwood, but these methods are not Equipment and other hardware requirements are high and limitations are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Obtaining the ITS Region Sequence of Dalbergia Dalbergia

[0028] 1. Sample DNA extraction

[0029] (1) Samples to be tested: Several samples of Dalbergia japonica were collected from different regions, as shown in Table 1.

[0030] Table 1 Collection locations of Dalbergia japonica samples

[0031] serial number sample Sampling site collection place 01 Dalbergia Dalbergia Sapwood Wencheng Town, Wenchang City, Hainan Province 02 Dalbergia Dalbergia Sapwood Beihe Town, Leizhou City, Guangdong Province 03 Dalbergia Dalbergia Sapwood Wushan Campus, South China Agricultural University, Guangzhou City, Guangdong Province

[0032] (2) According to the following method, the DNA of Dalbergia Dalbergia were extracted from the samples of Dalbergia Dalbergia in Table 1:

[0033] Soak the sample to be tested in 75% ethanol for 5 minutes, take it out, and place it in a sterile environment to air dry naturally.

[0034] (...

Embodiment 2

[0061] Embodiment 2 mahogany identification test

[0062] Three types of mahogany were purchased from the market, namely broad-leaved Dalbergia, Dalbergia and Brazilian black Dalbergia. After the sapwood was taken from the three samples, they were identified according to the following methods:

[0063] step 1

[0064] Soak the sample to be tested in 75% ethanol for 5 minutes, take it out, place it in a sterile environment to air dry naturally, take 2 g of the sample to be tested, add liquid nitrogen and grind it to powder, put it in a 10ml centrifuge tube, and then add 5ml of preheated 4×CTAB extract, mix well, and then take a water bath at 65°C for 2 hours, and shake gently every 15-20min; the formula of the 4×CTAB extract is: 4% CTAB, 0.1mol / L Tris -HCl, 1.4mol / LNaCl, 2%PVPP, 25mmol / LEDTA, high temperature and high pressure sterilization; among them, 2% β-mercaptoethanol is added after sterilization and cooling; "%" in the system represents the volume fraction.

[0065] Af...

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PUM

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Abstract

The invention discloses an ITS (internal transcribed spacer) sequence of dalbergia odorifera and a method for identifying the dalbergia odorifera by the ITS sequence. The ITS sequence of the dalbergia odorifera is characterized in that a nucleotide sequence is shown in SEQ ID NO:1. The method comprises the following steps of using primers ITS4 and ITS5 to perform PCR (polymerase chain reaction) proliferation on DNA (deoxyribonucleic acid) of a to-be-tested wood sample, so as to obtain ITS sequences; matching and comparing the two ITS sequences, and checking basic group information of ten sites, so as to judge whether the to-be-tested wood sample is the dalbergia odorifera or not. The method has the advantages that the dalbergia odorifera can be simply, quickly and efficiently subjected to molecular identification at the molecular biological level; the cost is low, the operation is simple, and the identification accuracy is high.

Description

technical field [0001] The invention relates to plant species identification technology, in particular to the identification technology of Dalbergia rosewood, in particular to an ITS region sequence of Dalbergia japonica and a method for identifying Dalbergia japonica using the ITS region sequence. Background technique [0002] Mahogany is a general term for a class of wood used to make mahogany furniture and mahogany crafts. According to the provisions of the national standard GB / T 18107-2000 "Redwood" of the People's Republic of China, there are 5 genera (Red Sandalwood, Dalbergia, Jasmine, Iron Sword Wood, Persimmon) and 8 categories (Red Sandalwood, Rosewood, etc.) 33 types of wood, fragrant wood, black rosewood, red rosewood, chicken wing wood, ebony, striped ebony). Among them, Dalbergia Dalbergia ( Dalbergia odorifera T. Chen) is an endangered species in Hainan and is a national second-class protected plant. It is commonly known as Hainan Xiangzhimu or Hainan Huang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 朱爽李奇威王业胜林月霞周林
Owner GUANGDONG PHARMA UNIV
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