Synthetic medium used for herba cistanche cells

Abstract

According to the invention, nutrient substance used for the growth of herba cistanche cells is widely selected through the experiment means, and the formula of the invention is determined according to the self metabolic properties and practical use effects of the herba cistanche cells. According to the formula, ammonium nitrate and potassium nitrate are taken as nitrogen sources, monopotassium phosphate is taken as phosphorus source, under the basic nutritional ingredients of the MS culture medium, the plant hormones including 2.4-D, 6-BA and NAA are added for the purpose of improving metabolites, meanwhile, the inducers including yeast extract and chitosan are added, through the above improvement, the growth rate of the herba cistanche cells is greatly improved, and the secretion amount of phenylethanoid glycosides is greatly increased. On the basis, according to the accidental discoveries, by adding the traditional Chinese medicinal materials with specific varieties and ingredients, the cell culture density can be improved. Based on the above beneficial discoveries, through the experiment means, the traditional Chinese medicinal materials used for treating the human diseases, such as herba tadehagis triquetri, herba saxifragae stoloniferae, herba ranunculi chinensis and herba abri are selected, and the obtained culture medium obtains the outstanding culture effects.

Description

technical field [0001] The invention relates to plant cell culture technology, and further relates to the design of nutritional conditions of plant cells, in particular to a synthetic culture medium for Cistanche deserticola cells. Background technique [0002] Plant cell culture technology is a culture process aimed at the expansion of cell mass and the accumulation of target metabolites based on isolated plant cells or protoplasts. For cell culture for the purpose of obtaining specific metabolites, the main process link is not only the expansion of cell mass, but also the induction of synthesis of target metabolites is more important for culture benefits. In the process of cultivating plant cells, culture conditions and nutritional conditions are the most important factors affecting yield. The culture conditions refer to the control of environmental conditions such as temperature, dissolved oxygen, light, and pH, while the nutritional conditions mainly refer to the culture...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a synthetic medium for Cistanche cells, so as to solve the technical problem that the Cistanche cell culture effect is not good in the prior art

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