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In-situ bioremediation preparation and remediation method for chlorothalonil contaminated soil

A technology for polluted soil and in-situ remediation, applied in the field of soil remediation, can solve the problems of photochemical degradation method being easily limited by light conditions, secondary chemical reagent pollution, etc. The effect of microecology

Active Publication Date: 2017-04-26
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the photochemical degradation method is easily limited by light conditions and easily causes secondary chemical reagent pollution to cultivated land in the remediation of chlorothalonil-contaminated soil, the present invention proposes a method for in-situ remediation of chlorothalonil-contaminated soil. Soil preparation and an in-situ bioremediation method for chlorothalonil-contaminated soil

Method used

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Examples

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Embodiment 1

[0052] This example is used to illustrate the preparation for in-situ remediation of chlorothalonil-contaminated soil provided by the present invention, and the method for in-situ remediation of chlorothalonil-contaminated soil using the preparation.

[0053] The composition of the preparation for in-situ remediation of chlorothalonil-contaminated soil provided in this example is as follows:

[0054] Aspergillus niger with the preservation number CGMCC No.11113, the content of which is 13 mg dry weight / mL;

[0055] Rhizomucor variabilis with preservation number CGMCC No.11114, with a content of 13 mg dry weight / mL;

[0056] Cunninghamellabertholletiae with the preservation number CGMCC No.11115, the content of which is 14 mg dry weight / mL;

[0057] The preservation number is Burkholderia (Burkholderia zhejiangensis) of CGMCC No.10848, the content is 10 15 a / mL;

[0058] The preservation number is Pseudomonas aeruginosa (Pseudomonasaeruginosa) of CGMCC No.10842, the content ...

Embodiment 2-16

[0065] This example is used to illustrate the preparation for in-situ remediation of chlorothalonil-contaminated soil provided by the present invention, and the method for in-situ remediation of chlorothalonil-contaminated soil using the preparation.

[0066] In the composition of the preparation that embodiment 2-16 provides for repairing chlorothalonil-contaminated soil in situ, except the content of the component in table 1-1 and 1-2, all the other components (microbial growth promoter and fertilizer ) content is all the same as in Example 1.

[0067]The preparations provided in Examples 2-16 for in-situ remediation of chlorothalonil-contaminated soil were evenly applied to the chlorothalonil-contaminated soil with a chlorothalonil content of 10 g / kg of soil, so that fungi and bacteria were removed from the soil Except that the content reaches the content shown in Table 2-1 and 2-2, the content of other components (microbial growth promoter and fertilizer) is all the same a...

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PUM

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Abstract

Relating to the soil remediation field, the invention discloses a preparation for in-situ remediation of chlorothalonil contaminated soil. The preparation contains fungi and / or bacteria with chlorothalonil degradation ability. The fungi can be at least one of Aspergillus niger, rhizomucor variabilis and Cunninghamella bertholletiae, and the bacteria can be at least one of Burkholderia zhejiangensis, pseudomonas aeruginosa and Stenotrophomonas maltophilia. According to the invention, the provided preparation is employed for in-situ bioremediation of chlorothalonil contaminated soil, not only overcomes the problems that photochemical degradation is easily limited by illumination conditions and can easily cause secondary chemical reagent pollution to arable land, at the same time makes full use of fungi and / or bacteria for catabolism of chlorothalonil residual in soil, thus solving the problem that high residual chlorothalonil in soil severely endangers food safety. At the same time, the micro-ecology of soil is improved, so that soil can recover farming ability more rapidly. Also, the method is low in cost, thus having broad application prospects.

Description

technical field [0001] The invention relates to the field of soil remediation, in particular to an in-situ bioremediation preparation and a remediation method for chlorothalonil-contaminated soil. Background technique [0002] Chlorothalonil is a protective stem and leaf spraying substituted benzene fungicide developed by Diamond Alkali Company of the United States. It is used in the prevention and control of various crop diseases such as vegetables, fruit trees, beans, rice, and wheat. However, due to the non-standard use and excessive spraying of chlorothalonil, a large amount of chlorothalonil remains on the surface of agricultural products and in the agricultural soil, which not only seriously endangers food safety, but also may damage the micro-ecological environment of the soil, causing the loss of agricultural soil fertility and crop disease. Hidden dangers. Currently, photochemical degradation is the main degradation pathway of pesticides. Studies by Penuela and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14B09C1/10C12R1/685C12R1/385C12R1/01C12R1/645
Inventor 任立伟唐丽伟林森王培红
Owner 粮华生物科技(北京)有限公司
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