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Acinetobacter bacteria NJAU-3 having functions of phosphate solubilizing and growth promoting, and applications thereof

A Bacillus bacterium, NJAU-3 technology, applied in the field of agricultural microorganisms, can solve the problems such as the unsatisfactory growth-promoting effect of Cucurbitaceae plants, and achieve the effects of promoting absorption and utilization, promoting growth, and improving growth

Active Publication Date: 2017-04-26
GENLIDUO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this bacterium has good growth-promoting effect on Solanaceae plants such as tomato, eggplant and other plants, but the growth-promoting effect on Cucurbitaceae plants such as cucumber is not ideal.

Method used

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  • Acinetobacter bacteria NJAU-3 having functions of phosphate solubilizing and growth promoting, and applications thereof
  • Acinetobacter bacteria NJAU-3 having functions of phosphate solubilizing and growth promoting, and applications thereof
  • Acinetobacter bacteria NJAU-3 having functions of phosphate solubilizing and growth promoting, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, isolation and identification of functional strains

[0026] Gently rinse the corn roots under tap water for 2 minutes to remove the sticky soil on the surface of the rhizosphere. Then rinse with sterile water, cut off the roots, put them into a 250mL conical flask with glass beads and 100mL of sterile water, shake at 25°C and 170r / min for 20min to form a soil suspension, and draw 10 -2 、10 -3 、10 -4 、10 -5 Dilute 0.1mL of the soil suspension, spread it on the inorganic phosphorus culture plate, and repeat each concentration 3 times. After culturing at 28°C for 5 days, select and purify the colonies that produce phosphorus-dissolving circles around the plate for more than 5 times. The colony of the phosphate-dissolving circle was transferred to the slant for culture, and stored at 4°C for later use.

[0027] The purified strains were inoculated on the improved PVK medium, placed in a 30°C incubator for 7 days, and the diameter of the transparent circle ...

Embodiment 2

[0034] Embodiment 2, the generation of functional bacteria suspension

[0035] Streak the NJAU-3 strains stored in glycerol tubes at -80°C on LB solid medium plates for activation, and cultivate them in a 37°C incubator for 12 hours. Pick a single colony of NJAU-3 in a 3ml liquid LB test tube, 37°C, 170r min-1 The culture was shaken for 10 hours, and the bacterial solution was used as the seed solution. Transfer the NJAU-3 seed liquid to the liquid LB shake flask with an inoculation amount of 1% (v / v), 37°C, 170r min -1 Cultivate to the mid-logarithmic phase (OD600=1.0), collect the bacteria by centrifugation at 4°C, wash the bacteria three times with distilled water, and resuspend with an equal volume of distilled water for use.

[0036] The preparation method of the LB culture medium used is as follows, taking the preparation of 1L medium as an example: peptone 10g, yeast powder 5g, NaCl 10g, agar 20g, constant volume to 1000mL, pH natural, sterilized at 121°C for 20min. ....

Embodiment 3

[0037] Embodiment 3, the application effect of biological seedling substrate on cucumber (first season, second season)

[0038] The seedling raising experiment with biological seedling substrate was carried out in the temperature net room of the National Organic Fertilizer Engineering Technology Research Center. The cucumber variety was Xinjinyan No. 7. The cucumber seeds were soaked in water at room temperature for 4 hours, placed on moist gauze and placed in a 30°C incubator to accelerate germination for 1 ~2d, after more than 75% of the seeds germinate, select the dew-white seeds and move them to the seedling tray for seedling cultivation. Three treatments were set up in the experiment: 1) CK, only distilled water was applied; 2) NJAU-3 was treated, and NJAU-3 bacterial suspension was applied; 3) Y40 was treated, and Y40 bacterial suspension was applied. Each handles 15 seedlings, each seedling tray is filled with 300g seedling substrate, and the amount of bacterial suspens...

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Abstract

The present invention provides a strain of Acinetobacter bacteria NJAU-3 having an effective crop growth promoting function, and applications thereof. According to the present invention, the strain is isolated from the Hebei corn field rhizosphere soil and is classified into the Acinetobacter sp bacteria based on the physiological and biochemical characteristics and the 16S rDNA analysis; the qualitative and quantitative analysis results show that the strain NJAU-3 has strong inorganic phosphorus solubilizing ability; and the seedling raising test results of the biological seedling raising matrix developed by using the strain show that the product developed based on the strain can effectively promote the growth of cucumber plants.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to an Acinetobacter growth-promoting bacterium NJAU-3 with phosphorus-solubilizing ability that can effectively promote crop growth and a biological seedling-raising substrate developed therefor. Background technique [0002] Phosphorus is one of the main nutrient elements that limit plant growth, and the crop utilization rate of the applied phosphorus fertilizer is 5% to 25%. Most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined with insoluble phosphate (Abd-Alla M H, 1994), most of the phosphorus fertilizers accumulate in the soil in an insoluble and ineffective state. Plant Growth-Promoting Rhizobacteria (PGPR) refers to microorganisms that live in the rhizosphere and root surface of plants and can directly or indirectly promote and regulate plant growth (Ahmad et al, 2008), among which phosphorus-solubilizing bacteria can Transfo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G9/10A01N63/02A01P21/00C05F11/08C12R1/01
CPCA01G24/00A01N63/10C05F11/08C12N1/205C12R2001/01C05F17/00
Inventor 沈其荣李静李荣张瑞福丁佳兴操一凡
Owner GENLIDUO BIO TECH CO LTD
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