Preparation method of cell nutrient solution for promoting hair regeneration

A nutrient solution and cell technology, which is applied in the field of human-derived cytokine hair growth nutrient solution and its preparation, can solve the problems of limited materials and inability to be used in clinical promotion, and achieve the effects of increasing survival, facilitating clinical promotion, and promoting cell secretion

Inactive Publication Date: 2017-05-10
陕西佰瑞衡健康科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for preparing a cell nutrient solution that promotes hair regeneration, which mainly solves the problem of hair loss that cannot be solved from the perspective of cell regeneration in the prior art, and at the same time, the hair growth components of the existing cell culture solution are limited due to limited materials Cannot be used for clinical promotion

Method used

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  • Preparation method of cell nutrient solution for promoting hair regeneration
  • Preparation method of cell nutrient solution for promoting hair regeneration

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Experimental program
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Effect test

Embodiment 1

[0034] Step 1: Primary isolation of ADSCs

[0035] The adipose tissue obtained by liposuction was centrifuged at 1500r / min at 21°C for 5min, and the liquid part was removed to obtain solid fat particles. Then remove the connective tissue from the obtained fat particles, cut the large pieces of tissue with tissue scissors, add an equal volume of normal saline to wash, centrifuge at 1000r / min, 21°C for 10min, remove the liquid part, and obtain solid fat particles. Then add collagenase solution prepared with an equal volume of normal saline, 200U / ml, 37°C, constant temperature shaking and digestion for 60min, collect the digestive juice, centrifuge at 800r / min, 21°C for 10min, remove the supernatant, take the cell pellet, and then add 5ml of normal saline , 800r / min, centrifuge at 21°C for 10min, wash the cells, take the cell pellet, resuspend with 3ml of culture medium, count and count at 1×10 6 cells / flask inoculated at 75cm 2 culture flask at 37°C, 5% CO 2 Cultivate in a co...

Embodiment 2

[0043] Step 1: Primary isolation of ADSCs

[0044] The adipose tissue obtained by liposuction was centrifuged at 2000r / min at 21°C for 3min, and the liquid part was removed to obtain solid fat particles. Then remove the connective tissue from the obtained fat particles, cut the large pieces of tissue with tissue scissors, add an equal volume of normal saline to wash, centrifuge at 1500r / min, 21°C for 3min, remove the liquid part, and obtain solid fat particles. Then add collagenase solution prepared with an equal volume of normal saline, 200U / ml, 37°C, shake and digest at a constant temperature for 30min, collect the digestive juice, centrifuge at 1000r / min, 21°C for 8min, remove the supernatant, take the cell pellet, and then add 8ml of normal saline , 1000r / min, centrifuge at 21°C for 8min, wash the cells, take the cell pellet, resuspend with 5ml of culture medium, count and count at 1×10 6 cells / flask inoculated at 75cm 2 culture flask at 37°C, 5% CO 2 Cultivate in a con...

Embodiment 3

[0052] Step 1: Primary isolation of ADSCs

[0053] The adipose tissue obtained by liposuction was centrifuged at 1000r / min at 21°C for 10min to remove the liquid part to obtain solid fat particles. Then remove the connective tissue from the obtained fat particles, cut the large pieces of tissue with tissue scissors, add an equal volume of saline to wash, centrifuge at 1200r / min, 21°C for 5min, remove the liquid part, and obtain solid fat particles. Then add collagenase solution prepared with an equal volume of normal saline, 200U / ml, 37°C, shake and digest at a constant temperature for 45min, collect the digestive juice, centrifuge at 1200r / min, 21°C for 5min, remove the supernatant, take the cell pellet, and then add 10ml of normal saline , 1500r / min, centrifuge at 21°C for 5min, wash the cells, take the cell pellet, resuspend with 10ml of culture medium, count and count at 1×10 6 cells / flask inoculated at 75cm 2 culture flask at 37°C, 5% CO 2 Cultivate in a constant tempe...

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Abstract

The invention provides a preparation method of a cell nutrient solution for promoting hair regeneration. The preparation method mainly comprises three steps: 1) primary isolation of ADSCs (adipose-derived stem cells); 2) enlarged cultivation of the ADSCs; and 3) induced culture of the ADSCs. The cell nutrient solution prepared by the invention can solve the problem of alopecia from the point of cell regeneration; the ADSCs-conditioned medium (the nutrient solution), in a periodic hair follicle reconstruction process, can activate a stem cell microenvironment of hair follicle and can regulate and control the normal growth of the hair follicle; by conducting low-dose UVB radiation, ADSCs survival, migration and paracrine capacities can be significantly improved, and an angiogenesis capacity and a hair regeneration capacity can be also obviously improved; by cultivating the ADSCs with a fibroblast-conditioned medium, cell survival and metabolism processes in a body environment can be simulated, and the secretion of more cell factors, which are conducive to cell microenvironment regulation and control, from cells can be promoted; and meanwhile, the cell nutrient solution prepared by the method is convenient in cell preparation, broad in source and convenient for clinical popularization, and cells can be derived from fat wastes obtained from clinical liposuction and skin grafts removed in other surgical operations.

Description

technical field [0001] The invention relates to a preparation method of a cell nutrient solution for promoting hair regeneration, in particular to a human-derived cytokine hair growth nutrient solution and a preparation method thereof. Background technique [0002] In recent years, with the increase of social competition and the deteriorating environment, the proportion of hair loss is on the rise, and the trend of younger people is obvious. At present, the medicines used to treat hair loss at home and abroad are mainly western medicines, such as minoxidil, which can expand the blood vessels under the scalp after being absorbed by the scalp, improve the microcirculation around the hair follicles, reduce the infiltration of inflammatory cells around the hair follicles, and thus enlarge the atrophic hair follicles And promote hair growth, so that vellus hair becomes terminal hair; in addition, finasteride is a drug that can inhibit the activity of 5α-reductase to prevent the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K8/99A61Q7/00
CPCA61K8/99A61Q7/00C12N5/0667
Inventor 李晓宁明磊国耿慧徐佳洁
Owner 陕西佰瑞衡健康科技有限公司
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