Kit for detecting monohydric phenol metabolites in urine and preparation method thereof

A technique for detecting monohydric phenols and detection reagents, which is applied in the field of kits for detecting monohydric phenol metabolites in urine and its preparation field, can solve problems affecting detection results, etc., achieve obvious color development effect, short detection time, The effect of high accuracy

Active Publication Date: 2019-01-15
江苏华鸣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of monohydric phenolic metabolites in urine is extremely small, and there are many other components in urine that will affect the detection results. Therefore, it is necessary to develop a more precise and specific The detection method is imminent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A test kit for detecting monohydric phenolic metabolites in urine, comprising an ampoule filled with detection reagents, the detection reagents being chitosan composite cellulose aerogels adsorbed with color-developing mixtures. Its preparation method is:

[0025] (1) Dissolve the natural cellulose with sodium hydroxide to obtain a 4wt% cellulose solution, then cast or coat the cellulose solution into a film, and prepare a fiber with a thickness of 20 microns by coagulation regeneration and water washing Plain film;

[0026] (2) casting or coating one deck chitosan solution on the cellulose film that step (1) prepares, the solvent that dissolves chitosan is acetic acid, and the chitosan concentration that prepares is 5wt%; After coagulating, obtain a Chitosan film with a layer thickness of 20 μm;

[0027] (3) Replace the cellulose-chitosan composite membrane obtained in step (2) with absolute ethanol, remove the moisture therein, and then reverse the cellulose-chitosa...

Embodiment 2

[0034] A test kit for detecting monohydric phenolic metabolites in urine, comprising an ampoule filled with detection reagents, the detection reagents being chitosan composite cellulose aerogels adsorbed with color-developing mixtures. Its preparation method is:

[0035] (1) Dissolve the natural cellulose with sodium hydroxide and urea to obtain a 6wt% cellulose solution, then cast or coat the cellulose solution into a film, regenerate by coagulation and wash with water, and prepare a thickness of 25 microns the cellulose film;

[0036] (2) casting or coating one deck chitosan solution on the cellulose film that step (1) prepares, the solvent that dissolves chitosan is acetic acid, and the chitosan concentration that prepares is 3wt%; Obtain a Chitosan film with a layer thickness of 25 μm;

[0037] (3) Replace the cellulose-chitosan composite membrane obtained in step (2) with absolute ethanol, remove the moisture therein, and then reverse the cellulose-chitosan composite me...

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Abstract

The invention discloses a kit for detecting a monohydroxyphenol metabolite in urine. The kit comprises an ampoule bottle accommodated with a detection reagent, wherein the detection reagent is chitosan compound cellulose aerogel adsorbed with a color development mixture. A preparation method comprises the following steps: preparing chitosan-cellulose-chitosan aerogel hierarchically, and then adsorbing tyrosinase, tryptophanase and a color development reagent which can react with an intermediate metabolite obtained by decomposing tyrosine and tryptophan and develop a color to the aerogel through an enzyme fixing method. The kit has a specificity detection function for the trace monohydroxyphenol metabolites in the urine, and a detection method is simple, quick and high in accuracy.

Description

technical field [0001] The invention relates to the technical field of tumor marker detection, in particular to a kit for detecting monohydric phenol metabolites in urine and a preparation method thereof. Background technique [0002] Tumor markers refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, which reflect the existence and growth of tumors, including proteins, hormones, enzymes ( isozymes) and oncogene products. Test the tumor markers in the patient's blood or body fluids to detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. [0003] Monohydric phenolic metabolites (ie, tyrosine, tryptophan, etc.) as tumor markers have been recognized by many scholars, such as CN102004103, Diagnostic value of rapid urine monohydric phenolic metabolite detection in malignant tumors ( Laboratory...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 余柯辰
Owner 江苏华鸣生物科技有限公司
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