A kit for detecting zeranol in foods

A technology of zearatol and detection kit, which is applied to measurement devices, instruments, scientific instruments and other directions, can solve the problems of expensive instruments and equipment, complicated operation, high requirements for operators, etc., and achieves the effect of simple operation and sensitive detection.

Inactive Publication Date: 2017-05-10
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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  • Abstract
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Problems solved by technology

[0003] At present, the determination methods of zearalanol mainly include high-performance liquid chromatography, gas chromatography, thin-layer chromatography, high-performance liquid chromatography-mass spectrometry, etc. The detection resu...

Method used

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Embodiment 1

[0020] In an embodiment of the present invention, a detection kit for zearallanol in food, comprising an enzyme label plate, a zearalanol standard product, a zearalenol antibody working solution, a zearalenol enzyme-labeled secondary antibody working solution, Substrate A solution, substrate B solution, stop solution, concentrated diluent and concentrated washing solution; substrate A solution is disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution containing 0.62mmol / L hydrogen peroxide; substrate Solution B is tetramethylbenzidinediamine solution; the stop solution is 2mol / L hydrochloric acid solution; the concentrated diluent is a 10-fold concentrated diluent, which is a 0.07mol / L phosphate buffer solution with a pH value of 7.3; concentrated washing The solution is a 10-fold concentrated washing solution, which is a phosphate buffer solution with a pH value of 7.4 containing 0.64wt.% Tween-20 and 0.014mol / L.

[0021] 1. Preparation of zearalenol antig...

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PUM

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Abstract

A kit for detecting zeranol in foods is disclosed. The kit includes an enzyme-linked immuno sorbent assay plate, a zeranol standard substance, a zeranol antibody working fluid, a zeranol enzyme-labeled secondary antibody working fluid, a substrate A solution, a substrate B solution, a stopping solution, a concentrated diluent and a concentrated washing liquid. Specific antibody competition between the zeranol in a sample and an antigen immobilized on the enzyme-linked immuno sorbent assay plate is utilized, the enzyme-labeled secondary antibody is added and reacted with the antibody, color developing occurs through an enzymatic color developing agent, and the zeranol content of the sample is determined according to the color developing shade. If the zeranol content of the sample is low, color is dark, and otherwise, color is light. A detecting method utilizing the kit is simple and convenient to operate, detection is sensitive, accurate and rapid and the kit is suitable for rapid detection of a large-scale of samples.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection kit for zearalanol in food. Background technique [0002] The residue of toxic metabolites in meat products has attracted more and more attention from the international community. In order to maximize the benefits, some unscrupulous traders illegally add zearalanol and other hormone drugs to animal feed to promote protein synthesis in animals. , improve feed utilization, thereby producing a role in promoting weight gain and obtaining more economic returns. Zearalanol and its metabolites have the biological activity of estrogens, and have inhibitory effects on gonadotropin-binding receptors and liver hormone-binding receptors in vitro. Residues of estrogens can cause dysfunction of human sex hormones and affect the normal development of secondary sexual characteristics. Under the induction of external conditions, they may cause cancer. [0003] At present, the...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/543
Inventor 张进吴念绮周朱晨张根义胡彬朱倩倩
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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