A kit for detecting zeranol in foods

Abstract

A kit for detecting zeranol in foods is disclosed. The kit includes an enzyme-linked immuno sorbent assay plate, a zeranol standard substance, a zeranol antibody working fluid, a zeranol enzyme-labeled secondary antibody working fluid, a substrate A solution, a substrate B solution, a stopping solution, a concentrated diluent and a concentrated washing liquid. Specific antibody competition between the zeranol in a sample and an antigen immobilized on the enzyme-linked immuno sorbent assay plate is utilized, the enzyme-labeled secondary antibody is added and reacted with the antibody, color developing occurs through an enzymatic color developing agent, and the zeranol content of the sample is determined according to the color developing shade. If the zeranol content of the sample is low, color is dark, and otherwise, color is light. A detecting method utilizing the kit is simple and convenient to operate, detection is sensitive, accurate and rapid and the kit is suitable for rapid detection of a large-scale of samples.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection kit for zearalanol in food. Background technique [0002] The residue of toxic metabolites in meat products has attracted more and more attention from the international community. In order to maximize the benefits, some unscrupulous traders illegally add zearalanol and other hormone drugs to animal feed to promote protein synthesis in animals. , improve feed utilization, thereby producing a role in promoting weight gain and obtaining more economic returns. Zearalanol and its metabolites have the biological activity of estrogens, and have inhibitory effects on gonadotropin-binding receptors and liver hormone-binding receptors in vitro. Residues of estrogens can cause dysfunction of human sex hormones and affect the normal development of secondary sexual characteristics. Under the induction of external conditions, they may cause cancer. [0003] At present, the...

AI Technical Summary

Problems solved by technology

[0003] At present, the determination methods of zearalanol mainly include high-performance liquid chromatography, gas chromatography, thin-layer chromatography, high-performance liquid chromatography-mass spectrometry, etc. The detection results are accurate and reliable, but the equipment is relatively expensive and the operation is difficult. It is cumbersome, time-consuming, and the sample pretreatment process is relatively complicated, which requires high requirements for operators