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Fructus lycii root rot-resistant bacillus tequilensis and application thereof

A technology of Bacillus tequila and Bacillus, applied in the application, bacteria, fungicides and other directions, can solve the problem of no Bacillus tequila, and achieve the effect of increasing species and strong antagonism

Active Publication Date: 2017-05-24
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bacillus tequilensis is a species in the genus Bacillus, which can produce a variety of polypeptide antibiotics and can produce antagonistic effects on a variety of plant pathogenic bacteria. The research on the biocontrol effect of Bacillus tequilensis mainly focuses on In the prevention and treatment of watermelon Fusarium wilt (CN105695368A), antagonizing G. mulberry (CN105274021A), and prevention and treatment of wheat head blight (CN104403962A), etc., but there is no report of Tequila bacillus for the prevention and treatment of Lycium barbarum root rot.

Method used

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  • Fructus lycii root rot-resistant bacillus tequilensis and application thereof
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  • Fructus lycii root rot-resistant bacillus tequilensis and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, screening of bacterial strain

[0029] (1) Isolation of strains

[0030] Five rhizosphere soil samples of healthy, dying and dead wolfberry trees were collected from Shilaba Village, Zhongning County, Ningxia Province. Weigh 10.0 grams of Lycium barbarum rhizosphere soil, add 90 mL of sterile water for every 10 grams of soil, put it into a pre-prepared conical flask filled with glass beads, place it on a constant temperature shaker at 37°C and shake at 180 rpm for 20-30 minutes. Gradient dilution method, the soil suspension was diluted to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 different gradients. Draw 0.1 mL of soil solution with appropriate dilution and place it on the LB plate medium, and spread it evenly (in this experiment, 10 -4 、10 -5 、10 -6 Three gradients were applied). Cultivate in a constant temperature incubator at 37°C for 24 hours, and pick a single colony. The obtained strains were purified by the three-section line method u...

Embodiment 2

[0047] Embodiment 2: the preparation of bacterial strain GQJK2 biocontrol agent

[0048] The specific preparation method is as follows:

[0049] The Bacillus tequilensis GQJK2 preserved on the slant and antagonizing wolfberry root rot was transferred to a test tube containing 5 mL of bean sprout juice liquid medium, and cultured in a constant temperature shaker at 37°C and 180 rpm for 12 hours. Then inoculate into 100mL / 250mL liquid bean sprouts juice culture medium according to 1% inoculum amount, put in 37 ℃, 180rpm constant temperature shaker and cultivate 24h, make concentration be 3 * 10 8 cfu / mL bacterial suspension.

[0050] Liquid bean sprout juice culture medium, its raw material composition is: 100g of soybean sprouts, 50g of sucrose, 1000mL of water, natural pH;

[0051] The preparation method is as follows: boil soybean sprouts for 30 minutes, filter with four layers of gauze, add sucrose to make the volume 1000mL.

Embodiment 3

[0052] Embodiment 3: field test

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Abstract

The invention discloses fructus lycii root rot-resistant bacillus tequilensis. The fructus lycii root rot-resistant bacillus tequilensis is classified and named as bacillus tequilensis GQJK2, which is collected in the China General Microbiological Culture Collection Center (CGMCC for short, located in the Institute of Microbiology, Chinese Academy of Sciences, #3, First Yard, Beichen Road West, Chaoyang District, Beijing) on November 10th, 2016, and the collection number of the fructus lycii root rot-resistant bacillus tequilensis is CGMCC NO. 13253. The resistant bacillus tequilensis GQJK2 obtained by isolation is relatively high in resistance to fructus lycii root rot, thus providing a novel biocontrol bacterium to biological prevention and control of the fructus lycii root rot and adding a variety to biocontrol preparations.

Description

technical field [0001] The invention relates to a bacillus tequila antagonizing wolfberry root rot and its application, belonging to the field of biotechnology. Background technique [0002] Lycium barbarum is a famous and precious traditional Chinese medicine. Lycium barbarum fruit has high nutritional, health care and medicinal value. It is also known as "four treasures of traditional Chinese medicine" together with Qiongzhen Ganoderma lucidum, Changbai Mountain ginseng and Dong'e Ejiao. With the development of the economy and the progress of the society, people are more and more aware of the value of wolfberry, prompting people to carry out extensive product development of wolfberry, and achieved important results. Ningxia wolfberry has the largest cultivation area in China. With the continuous expansion of wolfberry planting area, the incidence of wolfberry root rot in old production areas and new production areas has increased year after year, and it has become increasi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 杜秉海丁延芹汪城墙姚良同刘凯马锦锦
Owner SHANDONG AGRICULTURAL UNIVERSITY
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