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Lactose content detection method

A detection method and lactose content technology, which is applied in the detection of lactose content and the field of lactose detection, can solve the problems of unfriendly operators and the environment, and achieve the effect of separation and detection, less determination steps and reduced usage

Inactive Publication Date: 2017-05-24
ZHANGZHOU AONONG ANIMAL HUSBANDRY TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 3) The detection process needs to use a variety of reagents, including organic reagents and inorganic reagents, which are not friendly to operators and the environment

Method used

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  • Lactose content detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The instruments and chromatographic conditions used in this embodiment are as follows:

[0032] 1. Equipment

[0033] Electronic balance (accurate to 0.1mg), routine laboratory glassware, ultrasonic extractor, 0.22μm microporous filter membrane, solvent filter, high performance liquid chromatography with time difference shading detector, centrifuge.

[0034] 2. Chromatographic conditions

[0035] Column: Agilent Hi-Plex H(8μm, 7.7mm×300mm)

[0036] Column temperature: 70℃

[0037] Mobile phase: water

[0038] Injection volume: 20μL

[0039] A method for detecting lactose content, including the following steps:

[0040] Accurately weigh 0.1-5g of the sample to be tested, place it in a 100mL beaker, add 60mL of deionized water and stir evenly, ultrasonically extract at 60°C for 10min, transfer the solution to a 100mL volumetric flask, dilute to 100mL with deionized water, and take Centrifuge an appropriate amount of upper liquid at 8000r / min for 15min, take the supernatant after centr...

Embodiment 2

[0065] Another brand of feed was used, and the same operation steps as in Example 1 were used. The final results obtained are shown in Table 4.

[0066] Table 4 Test results of lactose content in samples to be tested

[0067] Actual value (%) Scalar addition (%) Recovery rate(%) National standard method (%) Relative deviation(%) Compound feed 0.210.1910.108.2

[0068] The invention can effectively reduce the time of pre-processing operations, save manpower and material resources, and save the use of toxic reagents. And can accurately detect lactose content.

Embodiment 3

[0070] Another brand of feed was used, and the same operation steps as in Example 1 were used. The final results obtained are shown in Table 5.

[0071] Table 5 Test results of lactose content in samples to be tested

[0072] Actual value (%) Scalar addition (%) Recovery rate(%) National standard method (%) Relative deviation(%) Concentrated feed21.91010611.95.3

[0073] The invention can effectively reduce the time of pre-processing operations, save manpower and material resources, and save the use of toxic reagents. Can accurately detect lactose content.

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Abstract

The invention discloses a lactose content detection method. The lactose content detection method comprises the following steps: (1) dissolving a to-be-detected sample into deionized water, and dissolving through ultrasonic waves to obtain a mixed solution; (2) transferring the mixed solution obtained in the step (1) to a volumetric flask to meter the volume, centrifugally filtering to obtain a to-be-detected solution; (3) repeating the method of the step (1) and the step (2), replacing the to-be-detected sample with a lactose standard product, and obtaining a standard solution; (4) using liquid chromatogram to detect the standard solution and the to-be-detected solution to obtain the retention time and the peak area of various components in the lactose standard product and the to-be-detected sample, drawing a corresponding standard curve for the peak area of the concentration of the lactose standard product, comparing the retention time and the peak area of the various components in the to-be-detected sample with the standard curve, and calculating to obtain the content of lactose in the to-be-detected sample. According to the lactose content detection method, the detection steps are few, the detection time is short, and the result is accurate.

Description

Technical field [0001] The invention relates to the technical field of chemical component detection, in particular to a method for detecting lactose content, in particular to a method for detecting lactose in animal feed and raw materials. Background technique [0002] Lactose is a disaccharide naturally found in mammalian milk. It is composed of one molecule of glucose and one molecule of galactose. For human children, lactose is a major nutrient in their growth and development. The development of intelligence plays a very important role. [0003] For the feed industry, lactose is also an important feed additive, especially for weaned suckling pigs. Newly weaned suckling pigs are facing many survival challenges, including their not yet fully developed digestive system. Facing the transition from breast milk to solid feed. At present, a large number of studies have shown that adding an appropriate amount of whey powder to the feed of weaning pigs can increase the feed intake, gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陈淋敏曾诚吴海英张书金周盛昌施建成肖丽萍周通杨伟春
Owner ZHANGZHOU AONONG ANIMAL HUSBANDRY TECH