A treatment method for improving the rate of rice radiation-induced healing distortion

Abstract

The invention discloses a treatment method capable of increasing the rice healing aberration generation rate by irradiation mutagenesis. The treatment method includes the steps of (1), exposing rice seeds to high-dose cobalt-60 gamma-ray to radiation; (2), subjecting the rice seeds obtained after the step (1) to microwave irradiation; (3), exposing the rice seeds obtained after the step (2) to low-dose cobalt-60 gamma-ray to radiation; (4), subjecting the rice seeds obtained after the step (3) to microwave irradiation; (5), cultivating the rice seeds obtained after the step (4) and performing excellent character selection, superior single selection and excellent resource and variety identification. The treatment method treats the rice seeds by combining cobalt-60 gamma-ray irradiation with microwave irradiation, so that the restriction that variation types are generated through single mutagenesis is overcome, and mutagenesis efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of rice mutagenesis, and in particular relates to a treatment method for accelerating the generation rate of rice radiation mutagenesis healing distortion. Background technique [0002] The main methods of rice breeding in my country are conventional hybridization method and cobalt 60 gamma radiation mutagenesis method. Some of these two methods are used in combination, and chemical and microwave mutagenesis are also used. In recent years, aerospace radiation mutagenesis method and transgenic breeding method have also been used. From the perspective of breeding, in order to quickly breed new varieties, first, the effect of mutagenesis is better, and second, the speed of stabilization is fast. The progeny of the conventional hybridization method is seriously separated, and it is extremely difficult to stabilize, which is an important reason for restricting its use. In contrast, the cobalt-60γ-ray radiation mu...

AI Technical Summary

Problems solved by technology

Irradiation of rice seeds with cobalt 60 gamma rays often adopts the semi-lethal dose radiation method, which is an empirical method. If the dose is too large, the DNA damage of the seed organism and the seeds will be too large, and the seed mortality rate will be too high to produce DNA Healing aberrations cannot be observed and realized. The radiation dose is too low to produce DNA healing aberrations or the proportion of aberrations is too small to achieve the purpose of mutagenesis