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Method for mutagenesis seedling of Chinese rose

A technology of rose and chemical mutagen, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of few variant varieties, high breeding costs, low degree of natural variation, etc., and achieve the improvement of rose variant varieties The effect of producing, reducing the cost of breeding, and increasing the probability of mutation

Inactive Publication Date: 2017-12-12
安徽徽思远生态农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the existing problems: new varieties of Chinese rose quality are hybridized, and hybrid rose seeds are obtained through natural pollination among varieties. The method for cultivating new varieties of this kind has a long cycle, low degree of natural variation, low probability of variety generation, and simple flower color; Although the single chemical mutagen is simple to operate and the mutagenesis rate is improved compared with pollination hybridization, there are fewer varieties of mutant varieties; while using tissue culture and normal temperature and pressure plasma mutagenesis technology, the equipment is complicated and the technical requirements are high. The cost is expensive, and it is difficult to promote and apply to ordinary rose growers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Seed pretreatment: first screen rose seeds with clean water to screen out low-quality seeds; soak the screened rose seeds in a 1:1000 potassium permanganate sterilizing solution for 12 minutes, and then soak them in clean water for 7 hours; After soaking the rose seeds, remove the pericarp bone layer of the seeds;

[0018] (2) Preliminary mutagenesis: Weigh isopropyl methanesulfonate, caffeine, 5-bromouracil and manganese chloride respectively according to the mass ratio, dissolve isopropyl methanesulfonate and 5-bromouracil In a small amount of ethanol, add water, caffeine and manganese chloride to prepare a chemical mutagen A at a concentration of 0.1%; pour the chemical mutagen A into the mutagenesis tank, which can be loaded with different voltages to induce The depth of the agent is 4-5cm, and the pretreated rose seeds are immersed in it for 8.5h; when the seeds are soaked, adjust the load voltage and current of the mutagenesis tank: first load voltage 12V, cur...

Embodiment 2

[0023] (1) Seed pretreatment: first use clean water to screen rose seeds to screen out low-quality seeds; the screened rose seeds are immersed in a 1:1000 potassium permanganate sterilizing solution for 14 minutes, and then soaked in clean water for 8 hours; After soaking the rose seeds, remove the pericarp bone layer of the seeds;

[0024] (2) Preliminary mutagenesis: Weigh isopropyl methanesulfonate, caffeine, 5-bromouracil and manganese chloride respectively according to the mass ratio, dissolve isopropyl methanesulfonate and 5-bromouracil In a small amount of ethanol, add water, caffeine and manganese chloride to prepare a chemical mutagen A at a concentration of 0.1%; pour the chemical mutagen A into the mutagenesis tank, which can be loaded with different voltages to induce The depth of the agent is 4-5cm, and the pretreated rose seeds are immersed in it for 9 hours; when the seeds are soaked, adjust the loading voltage and current of the mutagenesis tank: first load vol...

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Abstract

The invention discloses a method for mutagenesis seedling of Chinese rose. The method is characterized by comprising the following steps: (1) pre-treating seeds: sterilizing the seeds and removing peel sclerotin layer; (2) preliminarily mutating: preparing a mutagenic agent solution by using isopropyl methyl sulfonate, caffeine, 5-bromouracil and manganese chloride and then alternately loading 12V and 36V voltages on a mutagenesis slot; (3) performing low-voltage UV mutagenesis and suffering from three stages of low-voltage light intensity stage, low-voltage high light intensity stage and middle-voltage middle light intensity stage, wherein the vacuum jar pressure is at 0.2-0.8pa, the UV light strength is 120-290lux and the mutagenesis time is 20-22h; (4) culturing with nutrition solution; and (5) managing soil cultivation.

Description

technical field [0001] The invention belongs to the technical field of flower planting, and in particular relates to a method for raising seedlings by mutation of roses. Background technique [0002] Chinese rose, also known as periwinkle, Yueyuehong, etc., belongs to the family Rosaceae and belongs to the genus Rosa. Rose flowers are beautiful, varied, and fragrant. They can be used for ornamental purposes, widely planted in gardens and courtyards, and can also be used for medicinal and edible purposes. China is one of the origins of rose. It has rich flower colors and various flower shapes. Through cultivation and breeding methods, new varieties of roses can be cultivated without losing the true colors of roses. It has become a favorite flower variety for people. However, the cultivation of new varieties of rose generally adopts variety hybridization, single chemical mutagenesis and tissue culture mutagenesis, seed atmospheric pressure and normal temperature plasma mutage...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 杨天付
Owner 安徽徽思远生态农业发展有限公司
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