Breeding method of high-linoleic acid-content black soybean
A high linoleic acid, black soybean technology, applied in the fields of botanical equipment and methods, seed and rhizome treatment, plant genetic improvement, etc. Unpredictable effects, etc., to achieve the effect of improving linoleic acid content and genetic stability, improving cell differentiation and growth and development ability, and improving photosynthesis
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Embodiment 1
[0019] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:
[0020] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The γ-ray irradiation intensity is 73 rem for 15min / h, the time is 7.5h, and the γ-ray irradiation intensity is 115 rem for 12min / h, the time is 12.5h, and the irradiated seeds are obtained; the seed germination stage is relatively fragile, use Low irradiance, strong vitality after leaf bud development Using relatively high ultraviolet light can increase the probability of mutation;
[0021] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 3:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds, spray the fermented nutrient...
Embodiment 2
[0032] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:
[0033] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The intensity of gamma ray irradiation is 85 rem for 17min / h for 8h, and then the intensity of gamma ray irradiation is 120 rem for 14min / h for 13h to obtain irradiated seeds; the germination stage of seeds is relatively fragile, and low radiation Illumination, the vitality of the leaf buds is strong after development, and the use of relatively high ultraviolet light can increase the probability of mutagenesis;
[0034] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 4:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds,...
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