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Serum amyloid protein A/procalcitonin/C-reactive protein three-in-one determination kit and preparation method thereof
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A technology of reactive protein and serum starch, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of strong interference and high intensity, and achieve the effects of high sensitivity, fast response and easy operation
Inactive Publication Date: 2017-05-31
WEIHAI NEOPROBIO
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Embodiment 1
[0043] The various components of the test paper card in the serum amyloid A / procalcitonin / C-reactive protein three-in-one assay kit can be prepared by the following measures:
[0044] 1. Preparation of sample pad 2:
[0045] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours. 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:
[0046] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak at 4°C for 2 hours, then take it out of a 37°C oven and dry it for 4 hours, and set it aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the three pairs of serum amyloid A, procalcitonin and C-reactive protein labeled with rare earth fluorescent microspheres were injected using the Bio-Jet...
Embodiment 2
[0055] Embodiment 2: accuracy test
[0056]Select the above-mentioned test paper card and fluorescent immunochromatography analyzer (model: NEO-007), the setting of the parameters of the fluorescent immune analyzer: after setting the process parameters of the test paper card on the fluorescent immune analyzer, take the above-mentioned assembled test paper card , with 5, 20, 40, 60, 80, 100, 200 mg / L of SAA, 0.1, 1, 5, 10, 20, 30, 40 ng / mL of PCT, 0.5, 5, 20, 50, 100, 150 , 200mg / L CRP calibrator, measure with test paper card, get the fluorescence intensity value of each calibrator, input the result into the parameters of the analyzer, and complete the parameter setting of the analyzer.
[0057] Main testing materials: clinical samples obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, serum amyloid A / procalcitonin / C-reactive protein content distribution i...
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[0001] Technical field: [0002] The invention relates to the technical field of fluorescent immunochromatography in medical immunology, in particular to a method capable of rapidly and accurately detecting serum amyloid A, procalcitonin and C-reactive protein in serum, plasma and whole blood samples at the same time Serum amyloid A / procalcitonin / C-reactive protein three-in-one assay kit and preparation method for quantitative analysis of three infection and inflammation-related indicators. [0003] Background technique: [0004] Serum amyloid A (SAA) belongs to the acute phase protein of the human body and exists at a low level in the blood. When the human body is infected by bacteria or viruses, it rises rapidly after inflammation or active lesions or tissue damage. It can rise rapidly during the acute phase of inflammation or infection and decline rapidly during the recovery phase of the disease. Studies have shown that elevated serum SAA can be detected in diseases such as...
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