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Serum amyloid protein A/procalcitonin/C-reactive protein three-in-one determination kit and preparation method thereof

A technology of reactive protein and serum starch, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of strong interference and high intensity, and achieve the effects of high sensitivity, fast response and easy operation

Inactive Publication Date: 2017-05-31
WEIHAI NEOPROBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the sample and non-specific fluorescence emitted by proteins and other compounds in the serum) has a large intensity and interference. Strong, become the bottleneck of large-scale promotion of fluorescence analysis

Method used

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  • Serum amyloid protein A/procalcitonin/C-reactive protein three-in-one determination kit and preparation method thereof
  • Serum amyloid protein A/procalcitonin/C-reactive protein three-in-one determination kit and preparation method thereof
  • Serum amyloid protein A/procalcitonin/C-reactive protein three-in-one determination kit and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0043] The various components of the test paper card in the serum amyloid A / procalcitonin / C-reactive protein three-in-one assay kit can be prepared by the following measures:

[0044] 1. Preparation of sample pad 2:

[0045] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours. 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:

[0046] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak at 4°C for 2 hours, then take it out of a 37°C oven and dry it for 4 hours, and set it aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the three pairs of serum amyloid A, procalcitonin and C-reactive protein labeled with rare earth fluorescent microspheres were injected using the Bio-Jet...

Embodiment 2

[0055] Embodiment 2: accuracy test

[0056]Select the above-mentioned test paper card and fluorescent immunochromatography analyzer (model: NEO-007), the setting of the parameters of the fluorescent immune analyzer: after setting the process parameters of the test paper card on the fluorescent immune analyzer, take the above-mentioned assembled test paper card , with 5, 20, 40, 60, 80, 100, 200 mg / L of SAA, 0.1, 1, 5, 10, 20, 30, 40 ng / mL of PCT, 0.5, 5, 20, 50, 100, 150 , 200mg / L CRP calibrator, measure with test paper card, get the fluorescence intensity value of each calibrator, input the result into the parameters of the analyzer, and complete the parameter setting of the analyzer.

[0057] Main testing materials: clinical samples obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, serum amyloid A / procalcitonin / C-reactive protein content distribution i...

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Abstract

The invention relates to a serum amyloid protein A / procalcitonin / C-reactive protein three-in-one determination kit. The determination kit is provided with a test paper card and is characterized in that a PVC plate, a sample pad, a binding pad, a nitrocellulose membrane and a water absorbing pad are sequentially arranged on the test paper card from bottom to top, wherein the binding pad is adsorbed with three monoclonal antibody-microsphere coupling compounds, namely antiserum amyloid protein A, antiprocalcitonin, anti-C-reactive protein which are labeled by rare earth Eu<3+> fluorescent microspheres respectively, the diameter of the rare earth fluorescent microspheres is 150nm, and the rare earth fluorescent microspheres contain rare earth lanthanide element Eu<3+>, are stable in a basic state and transmit fluorescent light with the wavelength of 615nm under the action of an excitation light source at 337nm; and the monoclonal antibodies are purified mixed monoclonal antibodies which are respectively from monoclonal antibody cell strains specific to 2-6 different antigen epitopes such as serum amyloid protein A, procalcitonin and C-reactive protein. The determination kit provided by the invention has the advantages of simple and convenient operation, quick response, high sensitivity, strong specificity and the like.

Description

[0001] Technical field: [0002] The invention relates to the technical field of fluorescent immunochromatography in medical immunology, in particular to a method capable of rapidly and accurately detecting serum amyloid A, procalcitonin and C-reactive protein in serum, plasma and whole blood samples at the same time Serum amyloid A / procalcitonin / C-reactive protein three-in-one assay kit and preparation method for quantitative analysis of three infection and inflammation-related indicators. [0003] Background technique: [0004] Serum amyloid A (SAA) belongs to the acute phase protein of the human body and exists at a low level in the blood. When the human body is infected by bacteria or viruses, it rises rapidly after inflammation or active lesions or tissue damage. It can rise rapidly during the acute phase of inflammation or infection and decline rapidly during the recovery phase of the disease. Studies have shown that elevated serum SAA can be detected in diseases such as...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/543G01N33/533
CPCG01N33/6893G01N33/533G01N33/54313G01N33/558G01N33/577G01N2333/47G01N2333/4737G01N2333/585G01N2800/7095
Inventor 李红江于鸿翔刘衍亮陈萍萍宋璐琳王文亮
Owner WEIHAI NEOPROBIO
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