Application of tobacco C2H2-type zinc finger protein gene Nt540

Abstract

The invention discloses application of a tobacco C2H2-type zinc finger protein gene Nt540. A gene fragment with a nucleotide sequence shown as SEQ ID NO.1 is provided. The invention further discloses a recombinant vector comprising the gene fragment, a recombinant strain and application of the recombinant vector and the recombinant strain. Through transfer of the gene Nt540 and the recombinant strain, the tobacco quality is improved effectively, novel materials and novel approaches are provided for tobacco variety improvement, and accordingly such application has a promising application prospect.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the application of tobacco C2H2 type zinc finger protein gene Nt540. Background technique [0002] Tobacco is a special economic crop with smoking as the main purpose. Its aroma is one of the essential qualities of high-quality tobacco leaves. The specific performances are sufficient and pure, moderate strength, mellow taste, comfortable aftertaste, and high smoking safety. There are many factors that affect the aroma of tobacco leaves. Studies have shown that the epidermis of tobacco leaves can secrete essential oils, resins, waxes and other components. These substances play an important role in the insect resistance of tobacco, and are closely related to the aroma and taste of tobacco. According to reports, there are a total of 5289 chemical components stored in tobacco leaves and smoke. Among the many components, the substances that affect the quality of tobacco are mainly ...

AI Technical Summary

Problems solved by technology

[0003] Compared with the investment and achievements in the breeding of anti-virus, drought-resistant, and insect-resistant transgenic tobacco varieties, there are few researches on the application of genetic engineering technology in improving tobacco quality. At present, they mainly focus on specific substances such as terpenes, Carotene or potassium ions and other substances are regulated, but there are few studies on coordinating the carbon-nitrogen ratio of tobacco and reducing nicotine, especially on regulating the composition of tobacco leaves at the overall level to improve related quality.

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