A method for rapidly breeding seedlings of excellent strains of Sapindus
An excellent technology of Sapindus chinensis, which is applied in the field of rapid breeding of Sapindia chinensis excellent strain seedlings, can solve the problems that it is difficult to meet the demand for quantity and quality of Sapindus sativa seedlings, complicated and cumbersome operation process, time-consuming and labor-intensive survival rate, etc. Achieve the effect of saving germplasm material, simple operation method and convenient material collection
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Embodiment 1
[0032] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:
[0033] (1) Take materials
[0034] In July, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;
[0035] (2) Pretreatment of explants
[0036] The Sapindus branch in the step (1) is cut into 4.0cm long microstem section with 1 axillary bud and 1-2 leaf, after running water washing, by 1.0% (v / v) Tween and 0.4 % (w / v) Potassium permanganate mixed disinfection solution is carried out surface disinfection 10min, after pulling out, dry in the shade, standby;
[0037] (3) Induction treatment of explant adventitious roots
[0038] Place the morphology lower end of the micro-stem section after disinfection in step (2) in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO 4 ·7H 2 80...
Embodiment 2
[0044] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:
[0045] (1) Take materials
[0046] In June, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;
[0047] (2) Pretreatment of explants
[0048] The Sapindus branch in the step (1) is cut into 2.0cm long microstem section with 1 axillary bud and 1-2 leaf, after running water washing, by 0.5% (v / v) Tween and 0.1 % (w / v) Potassium permanganate mixed disinfection solution carried out surface disinfection for 5min, dried in the shade after being pulled out, and set aside;
[0049] (3) Induction treatment of explant adventitious roots
[0050] Place the morphology lower end of the micro-stem section after disinfection in step (2) in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO ...
Embodiment 3
[0056] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:
[0057] (1) Take materials
[0058] In June, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;
[0059] (2) Pretreatment of explants
[0060] The Sapindus branch in the step (1) is cut into 3.0cm long micro-stem segments with 1 axillary bud and 1-2 leaves, after running water washing, by 0.8% (v / v) Tween and 0.3 % (w / v) Potassium permanganate mixed disinfection solution carried out surface disinfection 8min, dried in the shade after being pulled out, standby;
[0061] (3) Induction treatment of explant adventitious roots
[0062] Place the morphology lower end of the micro-stem section after disinfection in step (2) in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO 4 ·7H...
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