The invention relates to a clonal
tissue culture breeding method for a
camphor tree and belongs to the technical field of forest tree
tissue culture. The clonal
tissue culture breeding method comprises the steps of explant material sterilization,
bud induction,
bud propagation, rooting induction, rooted-
seedling hardening, and
transplantation and management of tissue-cultured
seedling, and particularly comprises the following steps of: removing the leaves of an annual
shoot;
cutting the
shoot into stems which are respectively provided with 1-2 axillary buds; sterilizing the stems; carrying out induced culture by an inducing medium; carrying out
enrichment culture and rooting induction on the obtained new
bud; hardening and naturalizing the obtained rooted
seedling; and finally
transplanting the naturalized seedling into a matrix which is sterilized, and managing the transplanted seedling.
Seedling raising by the clonal tissue culture breeding method for the
camphor tree, disclosed by the invention, is not affected by natural factors such as season and weather, and besides, production cost is low,
land resources are saved, and seedling raising efficiency is improved; meanwhile, with adoption of the clonal tissue culture breeding method disclosed by the invention, the phenomenon of
browning can be effectively prevented, the effective
reproduction rate is high, the rooted seedling is grown regularly, the culture period is short, the
survival rate for the
transplanting of the tissue-cultured seedling is high, and the seedling stage culture period is short; and the clonal tissue culture breeding method is significant.