Rapid breeding method of koelreuteria paniculata

Abstract

The invention discloses a rapid breeding method of koelreuteria paniculata, and relates to the technical field of seedling culture of koelreuteria paniculata. The rapid breeding method is characterized by comprising the following steps: a, plant inoculation culture: preparing a tender stem with an axillary bud or a semi-lignified stem, disinfecting, cutting into stem segments of 1.0-1.5 centimeters, and inoculating onto a culture medium, wherein the temperature of a culture room is 23-25 DEG C; proliferation culture: when the axillary bud emerges to 1.0-2.0 centimeters in height after inoculation, cutting off a cluster bud block, inoculating into a differential medium to perform proliferation culture; root induction culture: taking a single robust test tube seedling which is 1-3 centimeters in height and preparing a 1 / 3 MS culture medium serving as a basic culture medium; acclimatization and transplanting: after a developed root system is formed on the test tube seedling, opening a bottle to perform acclimatization for 5-8 days, taking the seedling out, cleaning, disinfecting and transplanting into a culture medium. By using the method disclosed by the invention, excellent properties of koelreuteria paniculata and the consistence of nursery stocks are kept, and dependence on a large number of natural factors is lowered; the method has the advantage of rapid and mass breeding of high-quality varieties; the breeding coefficient is over three times that of the conventional seedling culture.

Description

Technical field: [0001] The invention relates to the technical field of nursery seedlings of the Luan tree, in particular to a method for rapid propagation of the Luan tree. Background technique: [0002] Koelreuteria paniculata (Koelreuteria paniculata), alias: Muluan, Luan Hua, etc., is a plant of the Sopinaceae and the genus Luan. It is a deciduous tree or shrub; the bark is thick, gray-brown to gray-black, longitudinally cracked when old; the lenticels are small, gray to dark and uncovered; the branchlets are warty points, and the leaf shafts and petioles are all wrinkled pubescent or Hairless. In the prior art, the seedlings of the Luan tree are all used for sowing and raising seedlings. Because the seed coat of the Luan tree seeds is hard and not easily permeable, if the germination management is not carried out, the second year spring sowing often does not germinate or the germination rate is very low. The germination rate is low, and the amount of seeds used should be l...

AI Technical Summary

Problems solved by technology

Therefore, there are many problems in the existing methods, such as long breeding period, low seed germination rate, large variation of offspring, and limitation of reproduction by natural conditions.