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A sheath-free particle three-dimensional focusing microfluidic chip and its focusing method

A microfluidic chip, focusing method technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., to achieve high-precision single-beam focusing, reduce stirring effects, and simple structure.

Active Publication Date: 2019-08-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although many types of microfluidic focusing techniques have been derived, none of the solutions can meet the above requirements at the same time.

Method used

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  • A sheath-free particle three-dimensional focusing microfluidic chip and its focusing method
  • A sheath-free particle three-dimensional focusing microfluidic chip and its focusing method
  • A sheath-free particle three-dimensional focusing microfluidic chip and its focusing method

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Embodiment

[0028] Embodiment: In a specific embodiment, the channel structure designed in the present invention can realize single-beam focusing on particle samples. B6-F10 black tumor particles and HeLa particle samples were diluted to 10000 / ml with PBS buffer (Thermo Fisher Scientific, MA, USA). Before passing the particle sample, pass PBS buffer solution into the flow channel to wash for 30 minutes. Afterwards, B6-F10 black tumor particles, HeLa particle suspensions, and polystyrene (PS) rigid balls with particle sizes of 7.32 μm, 10.4 μm, and 15.45 μm were passed into the flow channel at a flow rate of 0.8 ml / min for comparison. experiment. The flow channel has been washed with PBS buffer for 30 min before the particles are introduced. A MicroVision industrial camera was used to take continuous pictures of the outlet flow channel, and the exposure time was set to 10us. The 60 bright-field photos were superimposed using ImageJ software. The experimental results show that under the...

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Abstract

The invention relates to a sheath-liquid-free particle three-dimensional focusing microfluid chip and a focusing method thereof, and belongs to the field of inertial microfluid chips. The microfluid chip is formed by bonding encapsulation of a flow channel layer and a base layer, and the flow channel layer includes a flow channel structure high in depth-to-width ratio and formed by periodic alternation and series connection of rectangular-section straight flow channels and curved flow channels. In a curve of each focusing period, under the action of inertial lift force and Dean drag force, particles are pushed and squeezed to the center of the section of the flow channel together, and the particles are gathered to the outer side of the flow channel; by means of inertial lift force in the straight flow channels in the next focusing period, cross-section distribution difference, obtained in the previous period, of the particles is subjected to induction and reorganization, so that distribution density difference between the two sides of each straight flow channel is further increased, and after multi-period focusing cumulative effect, sheath-liquid-free particle single-beam three-dimensional focusing arrangement is achieved. By means of the flow channel structure, the three-dimensional single-beam focusing arrangement of particle groups with the flow speed range of 50-2000 microliter / min and the particle size range of 1-30 microns can be achieved.

Description

technical field [0001] The invention relates to the design and manufacturing method of a microfluidic chip for three-dimensional focusing arrangement of sheath-fluid-free particles, belonging to the field of inertial microfluidic chips. Background technique [0002] High-throughput sequencing and focusing of particle samples is an essential step in the clinical processing of many biological samples. Microfluidic technology provides an excellent technical platform for precise control of particle positions due to its advantages of light and small volume, small amount of samples / reagents used, fast speed, a large number of parallel processing, and powerful flow field control capabilities. At present, microfluidic particle focusing technology can be divided into two categories: sheath liquid focusing and sheath liquid focusing. Sheath liquid focusing uses one or more sheath liquid streams to squeeze the particle sample into a two-dimensional or three-dimensional fluid beam. Al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L2300/0877
Inventor 唐飞张岩王晓浩
Owner TSINGHUA UNIV
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