The present invention discloses a
time difference culture device, which relates to the technical field of
biological tissue culture; the
time difference culture box is adapted to be equipped with a
light source assembly, an observation
assembly and a
gas supply system; the
light source assembly includes a
first light focusing part, a light decaying sheet is arranged on the light incident side of the
first light focusing part, and the light decaying sheet is provided with a plurality of light transmitting parts in sequence along the length direction of the light decaying sheet, and the light
transmittance of the plurality of light transmitting parts increases in sequence along the length direction of the light decaying sheet; the observation assembly includes a
microscope camera and a
linear drive mechanism; the
gas supply system includes a first
pressure switch, a first reversing pressure
relief valve and a first gas
storage tank, the first
pressure switch is used to monitor the air pressure in the
time difference culture box and control the action of the first reversing pressure
relief valve, and the outlet of the first gas
storage tank is connected to the inlet of the first reversing pressure
relief valve after passing through a pressure reducing valve. The present invention can ensure the
clarity of the captured image, and the image has a three-dimensional sense and a sense of hierarchy, so as to accurately judge the morphology of biological tissues and ensure the accuracy of
biological tissue evaluation, analysis and screening.