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Lipase immobilized carrier, immobilized lipase and preparation method and application thereof

A technology of immobilized lipase and lipase, applied in the field of enzyme engineering, can solve the problems of high price and few varieties of immobilized lipase

Active Publication Date: 2022-07-05
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the specificity (especially Sn-1,3 specificity) immobilized lipases available in the market are few and expensive

Method used

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  • Lipase immobilized carrier, immobilized lipase and preparation method and application thereof
  • Lipase immobilized carrier, immobilized lipase and preparation method and application thereof
  • Lipase immobilized carrier, immobilized lipase and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0071] Preparation of immobilized lipase

[0072] The method of the present invention for preparing an immobilized lipase generally includes the steps of mixing the lipase with seed residue and powdered adsorbent, separating out the solid, and drying the solid.

[0073] It will be appreciated that the seed residue can be mixed with the powdered sorbent prior to mixing the resulting mixture with the lipase. Alternatively, it is also possible to mix the lipase with the seed residue or powdered sorbent first, and then mix the resulting mixture with another carrier (the powdered sorbent or the seed residue).

[0074] The mixing process is the immobilization process. It can be carried out in the manner of immobilization conventional in the art, for example, it can be mixed / immobilized in a mixer. For example, in certain embodiments, mixing is performed on an air bath shaker at 100-200 rpm. The mixing should be such that the lipase and the seed residue and / or powdered sorbent a...

Embodiment 1

[0099] Example 1: Preparation of immobilized lipase

[0100] 1. Mix 10g corn residue (Shanghai Kerry Food Industry Co., Ltd.) with 10g white carbon black (Hubo Engineering Materials Co., Ltd.), and 150mL self-fermented Thermomyces lanuginosus lipase solution (41700U / mL, measured by olive oil emulsification method) was added into it, the air-bath shaker was 120rpm, fixed at 25°C for 4h, the solid was collected by suction filtration, and the solid was divided into three parts for drying respectively, and the inlet air temperature of the fluidized bed was 40°C, Inlet air volume 0.6m 3 / min, fluidization time 35min, drying to obtain immobilized enzyme A, natural air-drying for 5 days to obtain immobilized enzyme B, and freeze-drying at -20°C for 24 h to obtain immobilized enzyme C.

[0101] 2. Mix 100g of bean dregs (Shanghai Kerry Food Industry Co., Ltd., water content 85%) with 10g of white carbon black (Hubo Engineering Materials Co., Ltd.), and mix the commercially available ...

Embodiment 2

[0111] Example 2: Immobilized lipase hydrolysis activity

[0112] The hydrolysis activity of the immobilized lipase was determined by the olive oil emulsification method, and the specific method was referred to the literature YT Gargouri, et al. Process Biochemistry, 2011. The results are shown in Table 2.

[0113] Table 2: Immobilized lipase hydrolysis activity

[0114] Enzyme number A B C D E F G H I Hydrolysis activity / kU / g 66 113 70 89 57 93 50 10 8

[0115] It can be seen from Table 2 that the immobilized lipase of the present invention has good hydrolysis activity.

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Abstract

The invention relates to a lipase immobilized carrier, an immobilized lipase and a preparation method and application thereof. Specifically, the present invention provides a carrier for immobilized lipase or an immobilized lipase using the carrier for immobilized lipase as a carrier, the carrier for immobilized lipase comprising seed residue and powder adsorbent, or an immobilized lipase containing seed residue and powder adsorbent Residue and powder sorbent composition. The present invention also relates to the use of the mixture of seed residue and powdered adsorbent in the preparation of immobilized lipase. The invention can reduce the cost of the immobilized lipase, and the prepared immobilized lipase has high activity.

Description

technical field [0001] The invention belongs to the field of enzyme engineering, and particularly relates to a lipase immobilized carrier, an immobilized lipase and a preparation method and application thereof. Background technique [0002] Lipase is a biocatalyst commonly used in industry and widely exists in animals, plants and microorganisms. Lipases can be divided into five categories according to their substrate specificity: (1) nonspecific lipases; (2) fatty acid-specific lipases; (3) position-specific lipases; (4) stereospecific lipases; (5) Substrate-specific lipase. The position-specific lipase preferentially catalyzes the Sn-1 and Sn-3 positions of triglycerides, so it is also called Sn-1,3-specific lipase. [0003] CN 1806044 B describes a preparation method of Sn-1,3 specific lipase powder. The method obtains immobilized enzyme by spray drying, and the enzyme liquid needs to undergo ultrafiltration treatment and adjust pH value before spray drying. The preparat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12P7/6454C12P7/6458
CPCC12N11/14C12P7/6418C12P7/6454
Inventor 李萌萌郑妍
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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