Auramine o rapid detection kit, detection method and application in detection of Chinese medicinal materials

A detection method and technology of traditional Chinese medicinal materials, which are applied in measurement devices, material separation, and analysis of materials, etc., can solve the problems of inapplicable on-site detection, complicated operation process, and high experimental cost, and achieve improved operation efficiency, simple operation and high sensitivity. Effect

Active Publication Date: 2018-12-21
GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there is no rapid detection kit for auramine O on the market. If the above-mentioned experimental method is used, the operation process is relatively complicated, the experimental time is long, the experimental cost is high, and it is not suitable for on-site detection.

Method used

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  • Auramine o rapid detection kit, detection method and application in detection of Chinese medicinal materials
  • Auramine o rapid detection kit, detection method and application in detection of Chinese medicinal materials
  • Auramine o rapid detection kit, detection method and application in detection of Chinese medicinal materials

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: detection object is Puhuang;

[0029] In this embodiment, whether auramine O is contained in Puhuang is quickly detected, and the specific steps are as follows:

[0030] Take about 1g of Puhuang and put it into a vial with 3ml of ethanol, tighten the cap, shake vigorously for about 30 seconds, let it stand for 1 minute, then shake for about 30 seconds, take a capillary tube, suck the solution to be tested, and Spot the sample in the center of the thin-layer plate, and the diameter of the liquid expansion is about 5-10mm. Repeat the spotting again after 1 minute. Observe that there are tender yellow spots in the central part of the thin-layer board, then it can be judged that the present embodiment contains auramine O composition in the cattail root.

Embodiment 2

[0031] Example 2: The detection object is Corydalis Corydalis.

[0032] In this embodiment, whether Corydalis contains auramine O is quickly detected, and the specific steps are as follows:

[0033] After the sample is crushed, take about 2g and put it into a vial with 5ml of ethanol; tighten the bottle cap, shake vigorously for about 30 seconds, let it stand for 1 minute, then shake for about 30 seconds, take a capillary tube, and get the sample to be tested. Spot the liquid in the center of the thin-layer plate. The diameter of the liquid is about 5-10 mm. Repeat the spotting once after 1 minute. Observe that there are bright yellow spots in the center of the thin-layer plate. It can be judged that Corydalis contains auramine O ingredients.

Embodiment 3

[0034] Implementation 3: The detection object is Phellodendron;

[0035] Whether the present embodiment contains auramine O in Cortex Phellodendri is quickly detected, and its specific steps are as follows:

[0036] After the sample is crushed, take about 5g and put it into a vial with 5ml of ethanol; tighten the bottle cap, shake vigorously for about 30 seconds, let stand for 1 minute, and then shake for about 30 seconds. Take a capillary tube to get the liquid to be tested, spot the sample in the center of the thin-layer plate, the diameter of the liquid is about 5-10 mm, and repeat the spotting again after 1 minute. Observe that there are tender yellow spots in the central part of the thin-layer board, then it can be judged that the present embodiment cork contains auramine O composition.

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Abstract

The invention provides an auramine O rapid detection kit, a detection method and application in detecting traditional Chinese medicinal materials. The kit comprises fittings and prepared liquid, such as an extracting solution, a thin-layer plate, a sample application tube, a centrifugal tube, a sucking tube, a penicillin bottle, a sampling spoon, water, alcohol or methanol; and the extracting solution is one or more of polar solvents such as water, alcohol and methanol. The detection method is simple to operate, has the advantages of convenience, no need of precise or large apparatuses, short detection time, and capability of generally completing detection within 4-6 minutes; moreover, detected results are visual, and judging can be performed by seeing color changes, so that the kit is very suitable for picking out fewer doubtful samples from a large quantity of samples, and greatly simplifies detection steps; precise detection such as mass spectrometry and chromatography is performed by only needing the fewer doubtful samples, so that detection cost is greatly reduced, and therefore, the kit is very suitable for quick screening needs of a supervision department, and has great social benefits of improving operation efficiency of the supervision department and quickly cracking down on criminals.

Description

technical field [0001] The invention relates to a rapid detection kit for auramine O, a detection method and an application in detection of Chinese medicinal materials. Background technique [0002] Auramine O, also known as base light yellow, canary yellow, Austrian yellow, basic Huaihuang, etc., is a chemical pigment, which is listed as a human carcinogenic compound by the International Cancer Research Institute (IARC), and is mainly used in acetate fiber and cotton fabrics Dyeing of paper, leather, paint, etc. Auramine O has a certain toxic effect on the human body, slightly irritating to the skin and mucous membranes, and can cause conjunctivitis, dermatitis, and upper respiratory tract irritation symptoms. Long-term excessive consumption will have harmful effects on the human body Kidney, liver damage and even cancer, the Ministry of Health listed it as a non-edible substance as early as 2008. [0003] Health preservation of traditional Chinese medicine has been a hot ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 杨鹏博马丽张陇清
Owner GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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