Vinasse fulvic acid and extraction method and application thereof

A technology of fulvic acid and distiller's grains, applied in the direction of chemicals, organic chemistry, and fungicides for biological control, to achieve the effect of inhibiting proliferation

Active Publication Date: 2017-07-21
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Vinasse fulvic acid and extraction method and application thereof
  • Vinasse fulvic acid and extraction method and application thereof
  • Vinasse fulvic acid and extraction method and application thereof

Examples

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Embodiment 1

[0054] Take a quantitative amount of distilled grains (with a moisture content of 63.67wt%) provided by Gansu Jinhui Wine Industry Group in a 250mL conical flask, add 50mL distilled water and shake well, continue to add 1mL 0.5mol / L sodium pyrophosphate before use 1mol / L NaOH adjusts the pH value. Shake well and place it in a 40℃ water bath for extraction for 20min, centrifuge at 3900r / min for 15min, take the supernatant and filter (-0.07MPa, room temperature), adjust the pH of the filtrate to 2 with 1mol / L HCl. After cold sinking at 10°C for 12 hours, centrifugation was performed at 3900 r / min for 15 minutes, and the supernatant was collected as the DFA extract. Rotary evaporation is carried out at 55°C and -0.07MPa, and after concentration, it is frozen at -20°C for 48h, and then placed in a freeze dryer for drying at -40°C and 0.0001MPa to obtain distillers fulvic acid (DFA).

Embodiment 2

[0056] Taking the mass ratio of white wine grains and distilled water as the solid-liquid ratio, the pH value was controlled to 11, and the vinasse fulvic acid was extracted according to the method of Example 1. In this example, the solid-liquid ratio was controlled to be 1:2.5, 1:5, 1:7.5, 1:10, 1:12.5, and 1:15 respectively, and the yield of lees fulvic acid was calculated. figure 1 As shown, figure 1 It is the effect of solid-liquid ratio on the yield of vinasse fulvic acid.

[0057] by figure 1 The results show that when the mass ratio of distillers grains to water is 1: (2.5-15), the yield of distillers grain fulvic acid is 4.1-5.3%, and as the amount of water increases, the extraction rate of DFA also gradually increases. When the liquid ratio is 1:7.5, the DFA yield is the highest, and then gradually flatten out.

Embodiment 3

[0059] Extract the lees fulvic acid according to the method of Example 1, control the solid-to-liquid ratio to 1:7.5, and control the pH values ​​of the alkaline distiller's lees to 8, 9, 10, 10.5, 11, 11.5, 12, 12.5, and calculate the lees fulvic acid Yield, the result is figure 2 As shown, figure 2 It is the influence of pH value on the yield of distillers grain fulvic acid.

[0060] by figure 2 The results show that when the pH is 8 to 12.5, the yield of lees fulvic acid is 4.9 to 5.65%. With the increase of pH value, the extraction rate of DFA increases first and then decreases. When the pH value is 11, the DFA yield is the highest. When the pH value is greater than 11, the decrease in the yield of DFA is due to the fact that after the alkali-soluble matter reaches saturation, more precipitates are precipitated during acid precipitation, which affects the yield of DFA.

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Abstract

The invention provides a vinasse fulvic acid extraction method. Firstly, vinasse is firstly regulated to be alkaline, and then hot extraction, acid analysis, precipitation and enrichment treatment are sequentially performed to extract vinasse fulvic acid. The experimental results of the embodiment show that vinasse fulvic acid can be successfully extracted from the vinasse by adopting the extraction method, and the yield of the vinasse fulvic acid is 4.2-6.25%. In addition, the obtained vinasse fulvic acid is a microcrystalline-state substance, is soluble in water, acid and alkali, is slightly soluble in ethyl alcohol, is not soluble in chloroform and acetone and does not produce an obvious layering phenomenon. The vinasse fulvic acid can also inhibit breast cancer cell MAD-231 proliferation except escherichia coli, bacillus subtilis, staphylococcus aureus, fusarium and alternaria alternate.

Description

Technical field [0001] The invention relates to the technical field of fulvic acid extraction, in particular to distiller's grain fulvic acid and its extraction method and application. Background technique [0002] The distiller’s lees is the mixed solids remaining after the fermented mash is distilled out of the wine. There are many unused protein, starch and fat in the distiller’s lees. At present, most of the solid distiller’s grains produced by our country’s wineries are used for firewood, fodder or agricultural fertilizers, but these utilizations have not made the best use of them. Because of the high water content of fresh distiller's grains, it will increase the cost of drying and transportation in utilization, resulting in a large amount of distiller's grains being randomly discarded or incinerated. If fresh distiller’s grains are not processed in time, they are prone to mold and deteriorate, which not only wastes valuable resources, but also pollutes the surrounding env...

Claims

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Application Information

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IPC IPC(8): C08H99/00C07G99/00A01P1/00A01P3/00A61P35/00
CPCC08H6/00C08H99/00C07G99/00
Inventor 赵萍芮文君邱悦王欢杨恒刘金涛
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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