Culture medium for endophytic fungi separation and rapid propagation of agilawood edgeworthia chrysantha

An endophytic fungus and culture medium technology, applied in fungi, separation of microorganisms, etc., can solve the problems of low growth factor concentration, arithmetic progression growth, affecting bacterial growth rate, etc. Simple, economical and practical effects

Inactive Publication Date: 2017-07-21
伍亭亦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the natural environment, the concentration of growth factors is extremely low. Once a part is used up by bacteria, it will affect the growth rate of bacteria in the next step, thus causing arithmetic progression growth.

Method used

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  • Culture medium for endophytic fungi separation and rapid propagation of agilawood edgeworthia chrysantha
  • Culture medium for endophytic fungi separation and rapid propagation of agilawood edgeworthia chrysantha

Examples

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preparation example Construction

[0015] The culture medium for the isolation and rapid propagation of the endophytic fungus of Agarwood sativa in the examples is prepared from raw material components comprising the following ratios by weight: 100-200 parts of potatoes, 15-40 parts of quinoa, 15-25 parts of glucose parts, 0-20 parts of agar, 700-900 parts of distilled water. The preparation method comprises the following steps:

[0016] (1) After peeling and slicing the potatoes, mix them with water at a weight ratio of 1:3-8, beat the pulp, separate the pulp and residue to obtain potato juice; or peel and cut the potatoes and add 3-8 times the weight of water Boil for 25-35 minutes, separate pulp and residue, and obtain potato juice;

[0017] (2) Soak the quinoa with 3-8 times the weight of water for 8-24h, beating, and separating the pulp and residue to obtain quinoa juice;

[0018] (3) Mix potato juice and quinoa juice, add glucose, agar (added when preparing solid medium) and distilled water, mix well, a...

Embodiment 1

[0021] A medium for the isolation and rapid propagation of endophytic fungi of Agarwood japonica, the preparation method comprising the following steps:

[0022] (1) Peel and slice the potatoes, weigh 200g and mix them with water in a weight ratio of 1:6, beat with a cell wall breaker, filter and separate the pulp residue, and obtain potato juice;

[0023] (2) Weigh 10g of quinoa and soak it in 6 times the weight of water for 12 hours, then beat it with a cell wall breaker, filter and separate the pulp residue, and obtain quinoa juice;

[0024] (3) Mix potato juice and quinoa juice, add 20g of glucose and 15g of agar, fully dissolve it, and dilute it to 1000ml with distilled water, mix well, adjust the pH to 5.8, and sterilize at 121°C for 20 minutes to obtain the product.

Embodiment 2

[0026] A medium for the isolation and rapid propagation of endophytic fungi of Agarwood japonica, the preparation method comprising the following steps:

[0027] (1) Peel and cut the potatoes, weigh 200g and add 8 times of water to boil for 30min, then beat with a cell wall breaking machine, filter and separate the pulp residue to obtain potato juice;

[0028] (2) Weigh 20g of quinoa and soak it in 5 times the weight of water for 16h, then beat it with a cell wall breaker, filter and separate the pulp residue, and obtain quinoa juice;

[0029] (3) Mix the potato juice and quinoa juice, add 20g of glucose and 10g of agar, fully dissolve it, and dilute to 1000ml with distilled water, mix well, adjust the pH to 6.0, and sterilize at 121°C for 20 minutes to obtain the product.

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Abstract

The invention belongs to the technical field of microculture, and provides a culture medium for endophytic fungi separation and rapid propagation of agilawood edgeworthia chrysantha, wherein the culture medium is prepared from potato, chenopodium quinoa, glucose, agar and distilled water. Meanwhile, the invention also provides a preparation method of the culture medium. The test result shows that the agilawood endophytic fungi quantity separated by the culture medium is obviously increased, and the agilawood endophytic fungi in fluid fungi shaking culture can reach the maximum growth within a short time, thereby greatly shortening the fungi shaking time. Moreover, all nutrition components of the culture medium are originated from the natural food materials (potato and chenopodium quinoa) and glucose; there is no any other additive; the culture of agilawood edgeworthia chrysantha fungi used as the edible treatment drugs is very important; the culture medium is green and environment-friendly, and the preparation is simple, economic and practicable.

Description

technical field [0001] The invention belongs to the technical field of microbial culture, in particular to a culture medium for isolating endophytic fungi of Agarwood and Agarwood and realizing rapid propagation, and is a green and environment-friendly culture medium suitable for the industrial production of Agarwood and Agarwood. The preparation method of the above-mentioned culture medium. Background technique [0002] Agarwood is the resinous wood of Aquilaria sinensis (Lour.) Gilg, a white woody tree of the genus Agarwood in the family Thymelae. Since ancient times, agarwood has been regarded as a kind of precious spice and Chinese herbal medicine. In the Song Dynasty, there was a saying of "one or two agarwood and one or two gold", which has high medicinal and commercial value. Agarwood has the effects of promoting qi and relieving pain, warming middle-JIAO and relieving vomiting, relieving qi and relieving asthma. It is distributed in Guangdong, Hainan, Guangxi, Fuji...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02
CPCC12N1/02C12N1/14
Inventor 伍亭亦
Owner 伍亭亦
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