Culture medium for endophytic fungi separation and rapid propagation of agilawood edgeworthia chrysantha
An endophytic fungus and culture medium technology, applied in fungi, separation of microorganisms, etc., can solve the problems of low growth factor concentration, arithmetic progression growth, affecting bacterial growth rate, etc. Simple, economical and practical effects
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[0015] The culture medium for the isolation and rapid propagation of the endophytic fungus of Agarwood sativa in the examples is prepared from raw material components comprising the following ratios by weight: 100-200 parts of potatoes, 15-40 parts of quinoa, 15-25 parts of glucose parts, 0-20 parts of agar, 700-900 parts of distilled water. The preparation method comprises the following steps:
[0016] (1) After peeling and slicing the potatoes, mix them with water at a weight ratio of 1:3-8, beat the pulp, separate the pulp and residue to obtain potato juice; or peel and cut the potatoes and add 3-8 times the weight of water Boil for 25-35 minutes, separate pulp and residue, and obtain potato juice;
[0017] (2) Soak the quinoa with 3-8 times the weight of water for 8-24h, beating, and separating the pulp and residue to obtain quinoa juice;
[0018] (3) Mix potato juice and quinoa juice, add glucose, agar (added when preparing solid medium) and distilled water, mix well, a...
Embodiment 1
[0021] A medium for the isolation and rapid propagation of endophytic fungi of Agarwood japonica, the preparation method comprising the following steps:
[0022] (1) Peel and slice the potatoes, weigh 200g and mix them with water in a weight ratio of 1:6, beat with a cell wall breaker, filter and separate the pulp residue, and obtain potato juice;
[0023] (2) Weigh 10g of quinoa and soak it in 6 times the weight of water for 12 hours, then beat it with a cell wall breaker, filter and separate the pulp residue, and obtain quinoa juice;
[0024] (3) Mix potato juice and quinoa juice, add 20g of glucose and 15g of agar, fully dissolve it, and dilute it to 1000ml with distilled water, mix well, adjust the pH to 5.8, and sterilize at 121°C for 20 minutes to obtain the product.
Embodiment 2
[0026] A medium for the isolation and rapid propagation of endophytic fungi of Agarwood japonica, the preparation method comprising the following steps:
[0027] (1) Peel and cut the potatoes, weigh 200g and add 8 times of water to boil for 30min, then beat with a cell wall breaking machine, filter and separate the pulp residue to obtain potato juice;
[0028] (2) Weigh 20g of quinoa and soak it in 5 times the weight of water for 16h, then beat it with a cell wall breaker, filter and separate the pulp residue, and obtain quinoa juice;
[0029] (3) Mix the potato juice and quinoa juice, add 20g of glucose and 10g of agar, fully dissolve it, and dilute to 1000ml with distilled water, mix well, adjust the pH to 6.0, and sterilize at 121°C for 20 minutes to obtain the product.
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