A method for purifying and inactivating egg yolk antibody
An egg yolk antibody and inactivation technology is applied in the field of purification and inactivation of egg yolk antibody, which can solve the problems of weak cellular immunity and low purity of egg yolk antibody, and achieve the effects of low preparation cost, good purity and convenient operation.
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Embodiment 1
[0033] The method for purifying and inactivating anti-gosling plague freeze-dried egg yolk antibodies comprises the following steps:
[0034] 1) Take 100 gosling plague-free eggs, disinfect and fumigate the eggs, beat the eggs by hand to separate the yolks;
[0035] 2) Add 3 times the volume of sterilized PBS buffer solution with a concentration of 0.01mol / L and pH 7.2 to the egg yolk, stir well, add sterile polyethylene glycol 6000, and stir while adding, so that the final concentration is 12% , continue to stir for 30 minutes, centrifuge at 3500 rpm for 10 minutes, and take the precipitate;
[0036] 3) Restore the precipitate to the volume of the original egg yolk with PBS buffer (same as above), then add 6 times the volume of acidified water (hydrochloric acid solution) pre-cooled to 2°C and pH 4.0, stir well, let stand at 4°C for 16 hours, and extract the Serum;
[0037] 4) Heating the supernatant to 22°C, and adding n-octanoic acid with a final concentration of 0.2% to ...
Embodiment 2
[0044] The purification and inactivation method of anti-porcine pseudorabies virus freeze-dried egg yolk antibody comprises the following steps:
[0045] 1) Take 100 porcine pseudorabies virus high-immunity eggs, disinfect and fumigate the eggs, beat the eggs by hand to separate the egg yolks;
[0046] 2) Add 2.5 times the volume of sterile PBS buffer solution with a concentration of 0.01mol / L and pH 7.2 to the egg yolk, stir well, add sterile polyethylene glycol 4000, and stir while adding, so that the final concentration is 15% , continue to stir for 30 minutes, centrifuge at 3000rpm for 15min, and take the precipitate;
[0047] 3) Restore the precipitate to the volume of the original egg yolk with PBS buffer (same as above), then add 5 times the volume of acidified water (hydrochloric acid solution) pre-cooled to 1°C and pH 4.2, stir well, let stand at 2°C for 20 hours, and extract the Serum;
[0048] 4) Heating the supernatant to 18°C, and adding n-octanoic acid with a f...
Embodiment 3
[0055] The method for purifying and inactivating the freeze-dried egg yolk antibody against Muscovy duck parvovirus comprises the following steps:
[0056] 1) Take 100 Muscovy duck parvovirus-free eggs, disinfect and fumigate the eggs, beat the eggs by hand to separate the yolks;
[0057] 2) Add 3.5 times the volume of sterile PBS buffer solution with a concentration of 0.01mol / L and pH 7.2 to the egg yolk, stir well, add sterile polyethylene glycol 8000, and stir while adding, so that the final concentration is 10% , continue to stir for 30 minutes, centrifuge at 4000rpm for 5min, and take the precipitate;
[0058] 3) Restore the precipitate to the volume of the original egg yolk with PBS buffer (same as above), then add 7 times the volume of acidified water (hydrochloric acid solution) pre-cooled to 4°C and pH 4.5, stir well, let stand at 8°C for 12 hours, and extract the Serum;
[0059] 4) Heating the supernatant to 18°C, and adding n-octanoic acid with a final concentrat...
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