Culture container and method for manufacturing culture container

Abstract

The present invention can provide a culture container capable of activating lymphocytes more efficiently than conventional ones, and a method for producing the same. The present invention is a bag-shaped culture container made of flexible packaging material for activating lymphocytes, and the inner surface of the opposite container is treated with 10-300ng / cm 2 The density is immobilized with anti-CD3 antibody, and the density per 1cm 2 The anti-CD3 antibody in the form of a solution is sealed in an amount of 0.25 to 400 ng and 0.1 to 800 μl on the culture surface composed of the above-mentioned one surface. In addition, in a bag-shaped culture container made of a flexible packaging material, the anti-CD3 antibody was immobilized at the above-mentioned density on the inner surface of the container facing each other, and the anti-CD3 antibody in solution was sealed in the above-mentioned amount.

Description

technical field [0001] The invention relates to a cell culture technique, in particular to a culture container for activating lymphocytes and a manufacturing method thereof. Background technique [0002] In recent years, in fields such as the production of pharmaceuticals, gene therapy, regenerative medicine, and immunotherapy, it is required to efficiently culture large quantities of cells, tissues, and microorganisms in an artificial environment. [0003] In such a situation, cells and a culture solution are enclosed in a culture container formed of a gas-permeable film, and a large number of cells are automatically cultured in a closed system. [0004] In particular, in the case of culturing lymphocytes, prior to culturing for increasing the number of cells, it is first necessary to perform culturing for activating lymphocytes using an anti-CD3 antibody. For this purpose, lymphocytes and a culture solution were added to a culture container containing an anti-CD3 antibody...

AI Technical Summary

Problems solved by technology

[0005] However, when only a small amount of solution-like anti-CD3 antibody is added to the culture container, the stimulation of lymphocytes is insufficient and activation cannot be performed sufficiently

On the other hand, when a large amount of solution-like anti-CD3 antibody is added to the culture container, the CD3 antigen present in the cell membrane will be shed due to the defense mechanism of the cells, and the activation of lymphocytes will be hindered.

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