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Method for establishing rat animal model with hypothyroid cardiac insufficiency

A technology of cardiac insufficiency and animal models, which can be applied to pharmaceutical formulations, in vivo radioactive preparations, preparations for in vivo experiments, etc., can solve problems such as not established, and achieve the effect of simple production, easy repetition, and low cost

Inactive Publication Date: 2017-08-04
河北省人民医院
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AI Technical Summary

Problems solved by technology

In order to better study the cause, pathological process and pathogenesis of hypothyroidism-induced cardiac insufficiency, it is very important to establish a suitable animal model. At present, no convenient and stable model of hypothyroidism-induced cardiac insufficiency has been established at home and abroad.

Method used

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  • Method for establishing rat animal model with hypothyroid cardiac insufficiency
  • Method for establishing rat animal model with hypothyroid cardiac insufficiency
  • Method for establishing rat animal model with hypothyroid cardiac insufficiency

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Experimental program
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Effect test

Embodiment

[0027] 1 Experimental materials

[0028] 1.1 Experimental animals

[0029] 56 clean-grade male healthy Wistar rats, weighing 200-220 grams, were purchased from the Experimental Animal Center of Hebei Province (license number: SCXK (Ji) 2013-1-003; certificate number: 1403051). Rats were reared in separate cages in the animal laboratory of Hebei Provincial People's Hospital, with free access to food and water, room temperature 20-25°C, relative humidity 40-70%, 12 hours of light per day, day and night cycle. Animal feed was common feed, which was purchased from the Experimental Animal Center of Hebei Province.

[0030] 1.2 Main reagents

[0031] 131 I preparation (Atom High-Tech Co., Ltd.), rat serum TT3ELISA kit, rat serum TT4ELISA test box, rat serum BNP ELISA test box (Sangon Bioengineering Co., Ltd.), rat serum TSH ELISA test box (Wuhan Ursen Technology Co., Ltd.).

[0032] 2 Experimental methods

[0033] 2.1 Establishment of animal models

[0034]Experimental groupi...

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Abstract

The invention discloses a method for establishing a rat animal model with hypothyroid cardiac insufficiency. The method comprises the following steps: A, diluting 131I with normal saline to prepare a mixed liquid; B, carrying out intraperitoneal injection on a rat with the mixed liquid; C, detecting at the end of four weeks and the end of eight weeks of the experiment to establish a model. According to the method, the thyroid iodine concentrating capability is understood through radioisotope scanning, and the levels of total triiodothyronine, total thyroxine and thyrotropin in serum are detected to evaluate the thyroid function, and the cardiac ultrasonography and brain natriuretic peptide of the rat are detected to evaluate the heart function. Detection data verifies that the intraperitoneal injection 131I caused rat animal model with hypothyroid cardiac insufficiency is successfully modeled.

Description

technical field [0001] The invention relates to the technical field of animal model construction methods, in particular to a method for establishing an animal model of hypothyroid cardiac insufficiency rats. Background technique [0002] The heart is an important target organ of thyroid hormones, and any change in the status of thyroid hormones will directly or indirectly affect heart function. In hypothyroidism, the secretion of thyroid hormone is insufficient, and non-specific pathological changes occur in the myocardium, resulting in the deposition of mucin and mucopolysaccharides in the interstitium of myocardial cells, myxedema, degeneration and even partial necrosis of the myocardium, resulting in myocardial fibrosis, resulting in a decrease in myocardial contractility , decreased cardiac output, and then cardiac insufficiency. In order to better study the cause, pathological process and pathogenesis of hypothyroidism-induced cardiac insufficiency, it is very importan...

Claims

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Application Information

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IPC IPC(8): A61K51/00A61K101/02
CPCA61K51/00
Inventor 魏立民赵春慧刘小娜王孟丹杜雪芹李宁王祥娜房京州
Owner 河北省人民医院
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