Method for establishing rat animal model with hypothyroid cardiac insufficiency
A technology of cardiac insufficiency and animal models, which can be applied to pharmaceutical formulations, in vivo radioactive preparations, preparations for in vivo experiments, etc., can solve problems such as not established, and achieve the effect of simple production, easy repetition, and low cost
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[0027] 1 Experimental materials
[0028] 1.1 Experimental animals
[0029] 56 clean-grade male healthy Wistar rats, weighing 200-220 grams, were purchased from the Experimental Animal Center of Hebei Province (license number: SCXK (Ji) 2013-1-003; certificate number: 1403051). Rats were reared in separate cages in the animal laboratory of Hebei Provincial People's Hospital, with free access to food and water, room temperature 20-25°C, relative humidity 40-70%, 12 hours of light per day, day and night cycle. Animal feed was common feed, which was purchased from the Experimental Animal Center of Hebei Province.
[0030] 1.2 Main reagents
[0031] 131 I preparation (Atom High-Tech Co., Ltd.), rat serum TT3ELISA kit, rat serum TT4ELISA test box, rat serum BNP ELISA test box (Sangon Bioengineering Co., Ltd.), rat serum TSH ELISA test box (Wuhan Ursen Technology Co., Ltd.).
[0032] 2 Experimental methods
[0033] 2.1 Establishment of animal models
[0034]Experimental groupi...
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