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Tissue culture method of fruits of lonicera fragrantissima subsp. standishii

A technology of tissue culture and branched fruit, which is applied in the field of plant cultivation, can solve the problems of short-term and large-scale rapid breeding of branched fruit, etc., and achieve the effect of remarkable rooting effect and accelerated growth

Active Publication Date: 2017-08-11
广西丰华农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Branches or branch cuttings are used for the traditional propagation of fork loquats, but this is still insufficient for short-term, large-scale rapid propagation of fork loquats.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of tissue culture method of fork loquat, comprises the following steps:

[0021] 1) Select a plant with good growth and thick fruit, cut off a 10mm stem section with buds, wash it with tap water, soak it in 70% ethanol solution for 30s, take it out and wash it with sterile water for 3 times, and dry it with sterile gauze , cut into 0.5cm 2 A small piece of the explant was obtained;

[0022] 2) Prepare a nutrient solution. The solute in the nutrient solution is 1.5 mg / L of 6-benzylaminopurine, 1 mg / L of 2,4 dichlorophenoxyacetic acid, and the solvent is 100 ml of sterile water. The obtained explants were put into nutrient solution, and the Erlenmeyer flask was placed on a reciprocating shaker for cultivation, and the shaker speed was set to 80r / min, and the nutrient solution was replaced every 2 days until new shoots grew;

[0023] 3) Put the explants that grow sprouts in step 2) into the subculture medium, the subculture medium components are: agar 10g / L, polyv...

Embodiment 2

[0028] A kind of tissue culture method of fork loquat, comprises the following steps:

[0029] 1) Select a plant with good growth and thick fruit, cut off a 10mm stem section with buds, wash it with tap water, soak it in 70% ethanol solution for 30s, take it out and wash it with sterile water for 3 times, and dry it with sterile gauze , cut into 0.5cm 2 A small piece of the explant was obtained;

[0030] 2) Configure the nutrient solution, add 1.5 mg / L of 6-benzylaminopurine, 1 mg / L of 2,4 dichlorophenoxyacetic acid, and 0.2 g / L of activated carbon into 100 ml of sterile water, put them into the Erlenmeyer flask, and put the The obtained explants were put into nutrient solution, and the Erlenmeyer flask was placed on a reciprocating shaker for cultivation, and the shaker speed was set to 80r / min, and the nutrient solution was replaced every 2 days until new shoots grew;

[0031] 3) Put the explants that sprouted in step 2) into the subculture medium, the subculture medium is...

Embodiment 3

[0036] A kind of tissue culture method of fork loquat, comprises the following steps:

[0037] 1) Select a plant with good growth and thick fruit, cut off a 10mm stem section with buds, wash it with tap water, soak it in 70% ethanol solution for 30s, take it out and wash it with sterile water for 3 times, and dry it with sterile gauze , cut into 0.5cm 2 A small piece of the explant was obtained;

[0038] 2) Prepare a nutrient solution, which consists of the following components: 6-benzylaminopurine 0.6mg / L, 6-glycosylaminopurine 0.6mg / L, IAA0.5mg, 2,4 dichlorophenoxyacetic acid 0.5mg / L , ascorbic acid 0.08g / L, gac 0.1g / L, garlic juice 15g / L, put into Erlenmeyer flask, the explant that step 1) obtains is put into nutrient solution, Erlenmeyer flask is placed on reciprocating shaker and cultivated , set the shaker speed to 80r / min, and replace the nutrient solution every 2 days until new shoots grow;

[0039]3) put the explants that sprouted in step 2) into the subculture med...

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PUM

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Abstract

The invention discloses a tissue culture method of fruits of lonicera fragrantissima subsp. Standishii. Totipotency of vegetable cells is utilized, plants which have good growth momentum and bear big fruits are adopted as explant, through the steps of induction culture, subculture, rooting culture, acclimatization and transplant and the like, the seedlings of the fruits of lonicera fragrantissima subsp. Standishii are obtained after culturing, the seedlings of the fruits of lonicera fragrantissima subsp. Standishii which have excellent genes can be cultured in large scale, the conditions like temperatures, illumination, nutrients in all culturing phases are controlled, and thus a large number of the plants of the fruits of lonicera fragrantissima subsp. Standishii. Totipotency of vegetable cells can be cultured within a short time.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for tissue culture of saffron. Background technique [0002] The fruit of the branched fruit is rich in nutrients, with a sugar-acid ratio as high as 39:1. The content of soluble sugar, soluble solids and vitamin C in the branched fruit is similar to that of common cultivated fruit trees, and the sugar content is significantly higher than that of prickly pear, seabuckthorn and kiwifruit. The content of mineral elements in the fruit is extremely high, and the content of calcium, iron, phosphorus, and amino acids is higher than that of other fruits, 24 times higher than that of apples, and the content of iron is 5 times higher than that of peaches. The content of phosphorus is the highest among all reported fruits. The most, and delicious and refreshing thick sweet aroma. In addition to the edible value, the branched loquat fruit has a small and exquisite plant shape, fra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈熙悌
Owner 广西丰华农业科技有限公司
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