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Curcuma total sesquiterpene and tri-sesquiterpene monomer and preparation method and application thereof

A technology of sesquiterpene and turmeric is applied in the field of total sesquiterpene and three sesquiterpene monomer compounds and preparation fields, which can solve the problems of sesquiterpene anti-diabetic activity and the like that are rarely seen in the genus Curcuma, and achieves expansion The effect of the drug's range of action

Active Publication Date: 2017-08-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since the 1980s, a lot of research work has been done on this genus of traditional Chinese medicine from the aspects of basic research and clinical application at home and abroad, and it is found that the rhizomes and tubers of Curcuma longa plants mainly contain sesquiterpene (volatile oil) and curcuminoids. , the pharmacological activities are mainly anti-tumor and anti-inflammatory activities, and there are few reports on the anti-diabetic activity of sesquiterpenes in curcuma

Method used

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  • Curcuma total sesquiterpene and tri-sesquiterpene monomer and preparation method and application thereof
  • Curcuma total sesquiterpene and tri-sesquiterpene monomer and preparation method and application thereof
  • Curcuma total sesquiterpene and tri-sesquiterpene monomer and preparation method and application thereof

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Effect test

Embodiment 1

[0029] Dry 10 kg of warm turmeric root, powder it, and extract it twice with 80 L of 95% ethanol solution at room temperature, each time for 5 days. Combine the extracts and steam until there is no alcohol smell to obtain 210g of the total extract. The total extract was dispersed with 2L of water, extracted with equal volumes of petroleum ether to obtain the CLP part of the petroleum ether extraction, and dried to obtain the total sesquiterpenes (98g). Then put the total sesquiterpenes on 200-300 mesh silica gel, and use petroleum ether-ethyl acetate system gradient elution (1:0-0:1), collect petroleum ether: ethyl acetate 50:1, 20:1, 10 : 1 eluted fraction, and then crystallized with n-hexane-acetone system to obtain compound 2 (2g), compound 3 (400mg) and compound 1 (4g) respectively.

Embodiment 2

[0031] 8.5 kg of dried turmeric rhizomes were powdered, refluxed with 85 L of ethyl acetate and extracted three times for 4 hours each time, filtered and combined to obtain a total extract of 900 g. The total extract was dispersed with 2L of water, extracted with chloroform, ethyl acetate, and n-butanol successively, and the chloroform fraction was obtained after the solvent was recovered, and 180 g of total sesquiterpenes were obtained after drying. Chloroform part extract was separated by 200-300 mesh silica gel column chromatography, petroleum ether-acetone (1:0-1:1) gradient elution, and petroleum ether-acetone (60:1), (30:1) were collected and (15:1) sites, the crude products of compound 2, compound 3 and 1 were obtained, respectively. Then recrystallized by dichloromethane-methanol system, the pure products of compound 2 (3g), compound 3 (100mg) and 1 (1g) could be obtained respectively.

Embodiment 3

[0033] Dried turmeric coarse powder 5kg, pulverized, added 40L dichloromethane, 80 degrees Celsius reflux extraction twice, the first time 2 hours, after the extract was filtered, added 40L dichloromethane to continue reflux extraction for 1 hour. The two extracts were combined and concentrated under reduced pressure to obtain 270 g of extract, which was suspended and dissolved in 2 L of water, extracted with equal volume of n-hexane, and dried to obtain 110 g of total sesquiterpenes.

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Abstract

The invention provides a curcuma total sesquiterpene and tri-sesquiterpene monomer as well as a preparation method and application on an aspect of resisting diabetes. A curcuma plant is taken as a raw material and is subjected to supercritical CO2 extraction or organic solvent extraction, then, low-polarity organic solvent is used for extraction to obtain a low-polarity total sesquiterpene extractive, and the low-polarity total sesquiterpene extractive is subjected to further chromatographic separation to obtain three types of sesquiterpene pure products of curcumenol, curdione and zedoary epoxy ketone. Activity research indicates that the total sesquiterpene, the curcumenol, the curdione and the zedoary epoxy ketone can be used for effectively increasing the consumption of glucose of HepG2 cells so as to obtain antidiabetic activity, and the total sesquiterpene, the curcumenol, the curdione and the zedoary epoxy ketone can be used for preparing antidiabetic medicines, antidiabetic health care food, functional foods and the like. The invention researches and develops new application of the diabetes resistance of the sesquiterpene in curcuma plants, the medicine function range of the curcuma plant is expanded, a basis is provided for carrying out popularization, utilization and deep processing on traditional Chinese medicinal materials, and a new application direction is provided.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and in particular relates to a total sesquiterpene and three sesquiterpene monomer compounds prepared from plants of the genus Curcuma longa and a preparation method thereof, as well as its application in the preparation of medicines with anti-diabetic effects, health food and Applications in products such as functional foods. Background technique [0002] Curcuma longa commonly used in traditional Chinese medicine includes turmeric, turmeric and zedoary. Turmeric is the dried rhizome of the plant Curcuma longa L.; Curcuma is Curcuma wenyujin Y.H.Chen et C.Ling, Curcuma longa L., Guangxi Zezhu (Curcuma kwangsiensis S.G.Lee et C.F.Liang) or Pengezhu (Curcumaphaeocaulis Val.), the first two are commonly called "Wen Yujin" and "Yellow Silk Curcuma" respectively, and the rest are commonly called "Osmanthus Curcuma" or "Green Silk Curcuma" according to their characters; Curcumaphaeocaulis V...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/08C07D307/93C07C49/607C07C45/78C07C45/79C07C45/82A61K31/343A61K31/352A61K31/122A61P3/10
CPCC07C45/78C07C45/79C07C45/82C07C49/607C07D307/93C07D493/08Y02P20/54
Inventor 甘礼社周长新莫建霞张丽莎吴昊姝
Owner ZHEJIANG UNIV
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