Probe assembly, gene capturing chip, kit and application thereof

Abstract

The invention provides a probe assembly, a gene capturing chip, a kit and application. The assembly at least comprises a probe specifically combined to a target gene under the strict conditions. A special capturing probe is designed for a targeted chemotherapy medicine related gene on the basis of the DNA next generation sequencing method, a plurality of target gene segments are obtained at a time through a small number of samples, high-depth sequencing is achieved through a high-flux sequencing platform, the genetic polymorphism of chemotherapy medicine and various deviant forms such as point mutation, insertion / deletion and DNA copy number alteration in targeted medicine related gene can be detected at a time in parallel, and therefore all target gene mutations related to targeted chemotherapy treatment are detected at a time. Thus, options are provided for clinical doctors in a real sense, a more suitable treatment scheme can be obtained only through single detection, and treatment cost and time are saved for patients to a larger extent.

Description

technical field [0001] The present invention generally relates to the field of biological detection, and specifically relates to a probe composition, a gene capture chip, a kit and applications thereof. Background technique [0002] Cancer is a complex disease caused by mutations in human genes. These gene mutations will cause cells to lose their normal regulatory functions, thereby proliferating indefinitely, and eventually leading to cancer. Targeted drugs can kill tumor cells carrying specific gene mutations, and have a significant effect. However, for different patients, the gene mutations carried in their bodies are also very different, which leads to the different sensitivity and side effects of each patient to the same anti-tumor drug. Studies have found that the killing effect of chemotherapy drugs on tumor cells is significantly related to the expression and / or polymorphism of a specific (group) gene; each chemotherapy drug has its corresponding evaluation target, ...

AI Technical Summary

Problems solved by technology

We found that there are two problems in the current gene detection products for precision medicine: first, the detection methods focus on the detection of genes related to targeted drugs, while the genetic detection of chemotherapy drugs is rarely involved; second, the gene detection of targeted drugs designed for a single cancer The detection products only focus on the drugs approved by FDA / CFDA for this type of cancer, and the first-level drug guidance lacks guidance on potentially available drugs, and there is little information on drug selection available to clinicians

[0006] The clinical routine drug detection of cancer patients is still in the "testing + treatment" mode of "chemotherapy detection-chemotherapy 1-chemotherapy 2-targeted detection-targeted therapy", which lasts for a long time and cannot quickly get the best treatment. treatment plan, delaying the best treatment period, and greatly increasing the cost of treatment in the process of continuous treatment plan replacement

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