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Tissue culture rapid propagation method of Chinese herbal medicine, namely piper wallichii

A technology of tissue culture rapid propagation and heather vine, which is applied in the field of plant tissue culture, can solve the problems that there is no patent application for heather tissue culture rapid propagation, and there is no heather tissue culture technology, so as to promote industrial development Utilization, low cost, strong technical effect

Inactive Publication Date: 2017-09-01
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of Photinia vine at home and abroad, and there is no application for a patent for tissue culture and rapid propagation of Photinia vine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In the field, select the middle and upper part of the heather vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time .

[0020] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, rinsed with sterile water for 5 seconds After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 15 minutes, wash it with sterile water for 5 times, dry it with sterile filter paper, and cut it into 1.5-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 25° C. for 45 days in full dark culture to induce t...

Embodiment 2

[0026] (1) Collection of explants: In the field, select the middle and upper part of the heather vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time .

[0027] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 18 seconds, rinsed with sterile water for 6 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 17 minutes, wash it with sterile water for 6 times, dry it with sterile filter paper, and cut it into 1.5-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 26° C. for 53 days in full dark culture to induce the forma...

Embodiment 3

[0033] (1) Collection of explants: In the field, select the middle and upper part of the heather vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time .

[0034](2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 20 seconds, rinsed with sterile water for 7 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 20 minutes, wash it with sterile water for 5 to 7 times, dry it with sterile filter paper, and cut it into strips of about 1.5 to 2.5 cm. Canes were inoculated into the induction medium, placed at 28°C for 60 days in total darkness to induce the format...

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PUM

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Abstract

The invention relates to a method of plant tissue culture in agricultural biotechnology, in particular to a tissue culture rapid propagation method of a Chinese herbal medicine, namely piper wallichii. The method comprises the following steps which are carried out successively: obtaining an explant of the piper wallichii, carrying out induction culture on the explant, proliferation culture and rooting culture and transplanting; an explant induction culture medium operated in the explant induction culture comprises a White culture medium, 2.0 to 4.0mg / L of KT, 0.5 to 1.0mg / L of IBA, 20 to 30g / L of sucrose, 3.5 to 4.0g / L of agar, and 0.1 to 0.3g / L of activated carbon, and the pH of the explant induction culture medium is 5.4 to 5.8, according to the invention, stem segments of the piper wallichii are taken as the explant, and subjected to the steps of induction, proliferation, rooting, seedling hardening, transplanting and the like, the induction rate of the piper wallichii reaches more than 89%, the survival rate of the piper wallichii reaches more than 95%, and the tissue culture rapid propagation of the Chinese herbal medicine, namely the piper wallichii is realized to achieve the purpose of the invention.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of traditional Chinese medicine heather vine. Background technique [0002] Heather vine [Piper wallichii (Miq.) Hand.-Mazz] is an evergreen climbing vine of the family Piperaceae. Its dry part above the ground is used as medicine. It is slightly warm in nature and bitter in taste. It has the effects of dispelling rheumatism, strengthening the waist and knees, nourishing the kidney and strengthening yang, relieving cough and asthma, promoting blood circulation and relieving pain. Treatment of dysmenorrhea, bruises, swelling, pain and other diseases. In my country, heather vine is mainly found in Guangxi, Hubei, Hunan, Guizhou, Yunnan, Sichuan and other provinces and regions. It is often found in the shade or wet places in the forest, and climbs on stone walls or trees. At present, heather vine is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞莫昭展杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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