Liaofu variety big fruit sea-buckthorn shoot tip tissue medium formula
A technology of tissue culture medium and seabuckthorn, which is applied in the field of plant tissue culture, can solve the problems of no industrial production reports and formulas, and achieve the effect of convenient and rapid propagation
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[0014] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.
[0015] A kind of Liaofu cultivar Seabuckthorn shoot tip tissue culture medium formula, including three kinds of primary culture medium, callus induction medium and subculture medium, wherein,
[0016] Formula of primary medium: 1 / 2MS, 6-BA0.5mg / L, sucrose 30g / L, agar 6g / L; when preparing, first measure MS medium, add 6-BA, add sucrose, after the sucrose is dissolved, Adjust the pH to 5.6-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121° C., 25 minutes), and cool to a solid for later use.
[0017] Callus induction medium formula: 1 / 2MS, 6-BA0.5mg / L, IBA0.5mg / L, sucrose 30g / L, agar 6g / L; when preparing, first measure MS medium, add 6-BA , IBA, add sucrose, after the sucrose is dissolved, adjust the pH to 5.6-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121°C, 2...
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