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Method for detecting drug resistance of chilo suppressalis (Walker)

A technology of Chilo borer and insecticide resistance, applied in the field of chemical pest control, can solve the problems of cumbersome operation and no improvement, and achieve the effect of simple test operation, simple equipment and good practicability

Inactive Publication Date: 2017-09-15
INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional drip method, rice seedling dipping method and artificial feed drug film method are cumbersome to measure the toxicity, and it is necessary to set several concentrations and repeat to ensure the reliability of the results.
The artificial feed dipping method is a new method for detecting the resistance of Chilo borer invented by our laboratory. It has the advantages of simple operation, fast and accurate, and can fully reflect the toxicity and activity of various agents. However, the concentration and repeated The problem is still the same as the traditional method, no improvement

Method used

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  • Method for detecting drug resistance of chilo suppressalis (Walker)
  • Method for detecting drug resistance of chilo suppressalis (Walker)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtaining of embodiment 1 diagnostic dosage

[0026] 1. Research object

[0027] The research object of the present invention is the sensitive Chilo suppressalis population that has been fed for more than 40 generations with artificial feed and screened for 3 generations by single egg mass screening without contacting any chemical agent.

[0028] 2. Method

[0029] 2.1 Drugs to be tested: triazophos, abamectin and chlorantraniliprole were dissolved and diluted with dimethylformamide to 5-7 different concentrations as the concentration for toxicity determination; dissultap was dissolved and diluted with distilled water 5-7 different concentrations are used as concentrations for toxicity determination.

[0030] 2.2 Preparation of artificial feed:

[0031] ① Preparation of Phase A: Add 135 grams of wheat germ powder, 120 grams of sucrose, 45 grams of cellulose, 155 grams of casein and 20 grams of yeast powder into 1000 ml of water, stir well and place in an automatic st...

Embodiment 2

[0040] Example 2 Diagnostic Dosage Detection of Resistance to Field Populations of Chilo suppressalis

[0041] 1 detection object

[0042] The detection object is the field population of Chilo borer to be tested. The eggs of Chilo suppressalis were collected from the field, and the hatched Chilo borer larvae were raised on artificial feed until the 4th instar to be tested.

[0043] 2 methods

[0044] The test agents triazophos, abamectin, chlorantraniliprole and monosultap were used to detect the resistance of the field population of Chilo borer by using the diagnostic dosage of the present invention.

[0045] 2.1 Configuration and dilution of the mother liquor of the original drug

[0046] Dissolve and dilute triazophos, avermectin and chlorantraniliprole with dimethylformamide to obtain a diagnostic dose for use, and dissolve and dilute the original drug for monosultap with distilled water to obtain a diagnostic dose for use.

[0047] 2.2 Preparation of artificial feed: ...

Embodiment 3

[0053] Example 3 Comparison of the diagnostic dosage method of artificial feed soaking medicine and the traditional drip method for the determination of resistance to the field population of Chilo suppressalis

[0054] The traditional instillation method requires precise instillation instruments, and the operation steps are cumbersome, and the steps are repeated for each bioassay, and a large amount of insects are used. By adopting the diagnostic dose of the present invention, artificial feed with medicine is prepared, and the amount of insects used is small, and the drug resistance of field populations can be detected simply and quickly.

[0055] The tested populations of Chilo suppressalis were: Nanchang County population, Dayu County population and Poyang County population in Jiangxi Province. The LC of 3 tested populations to 3 pesticides (triazophos, abamectin and monosultap) was determined by spot method. 50 , see Table 2. Simultaneously adopt the diagnostic dosage of ...

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Abstract

The invention provides a method for detecting drug resistance of chilo suppressalis (Walker). According to the method, a drug-carried artificial feed piece is prepared on the basis of diagnosis dosages of an artificial feed drug soaking method, and the drug resistance of field populations of the chilo suppressalis (Walker) is rapidly detected. The drug comprises the following components in diagnosis dosages: 1.502mg / L of abamectin, 38.8334mg / L of monosultap, 20.9033mg / L of triazophos and 20.2148mg / L of chlorantraniliprole. The method provided by the invention is simple and convenient to use, the toxicity and the activity of the drug can be sufficiently reflected, a small amount of insects is needed, the defects that a conventional bioassay method is large in insect amount, tedious to operate, great in time and labor and the like are overcome, and the drug resistance of the field populations of the chilo suppressalis (Walker) can be rapidly detected.

Description

technical field [0001] The invention relates to the field of pest chemical control, in particular to a method for detecting the resistance of Chilo borer. Background technique [0002] Chilo suppressalis (Walker), belonging to Lepidoptera Boreridae, is one of the important pests of rice, which can cause symptoms such as withered sheath, dead heart, dead booting, white ears and insect damage to rice, and is harmful to rice production. cause heavy losses. In recent years, due to changes in climate, farming systems and cultivation methods, the population of Chilo suppressalis has shown a rapid upward trend. Especially after 1998, it has become the primary pest in the Yangtze River Basin and Jiangnan rice fields, seriously hindering the stable and high yield of rice. For a long time, the control of chemical pesticides has been the main measure to control the damage of the stem borer. However, due to the long-term unreasonable use of the pesticides, the stem borer has developed ...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 黄水金孙杨秦文婧秦厚国
Owner INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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