The invention discloses a
rapid detection platform and method of PD-L1
exosome in vitro, and belongs to the technical field of
rapid detection. The detection platform comprises a sample initial-
filtration area, a
nanofiltration area, a coloration object pad area, a coloration area and a water absorption area. In a
capture antibody on the coloration area and a coloration object labeled
antibody onthe coloration object pad area, at least one is an exosomePD-L1
antibody. The detection method comprises the steps that a to-be-tested sample is filtered initially through an initial-filtering film, then the to-be-tested sample is filtered through a
nanofiltration film, and thus the
exosome is obtained; the
exosome is specifically combined with the coloration object labeled
antibody and then combined with the
capture antibody to form a double-antibody sandwich; and in the
capture antibody and the coloration object labeled antibody, at least one is the exosome PD-L1 antibody. According to the
rapid detection platform of the PD-L1 exosome
in vitro,
whole blood separation, exosome purifying and special detection of the PD-L1exosome are integrated by the platform, and the characteristics thatthe operation is convenient, detection is fast, and the cost is low are achieved.