Magnetic particle chemiluminescence immunoassay analyzer multi-channel detection method

Abstract

The invention discloses a multi-channel parallel method for a magnetic particle chemiluminescence immunoassay analyzer. Fill the cup with detection reagent and transport it to the reagent mixing channel; transport it to the incubation system to incubate the test sample in the reaction cup; transport it to the washing channel for washing; transport the cup strip to the substrate filling and mixing channel for filling Substrate; transported to the detection waiting channel for detection; grasping the reaction cups in the detection waiting channel to the detection system one by one for detection; There are at least two washing channels. The invention greatly improves the detection efficiency while ensuring the detection flexibility, and the cup strip can be recycled and reused, the consumption of consumables is small, and the detection cost is low.

Description

technical field [0001] The invention relates to a multi-channel detection method for a magnetic particle chemiluminescence immunoassay analyzer, belonging to the technical field of detection and analysis. Background technique [0002] Magnetic particle luminescence immunoassay is a detection method combining luminescence immunoassay method and magnetic particle separation technology. Because magnetic particles have the characteristics of magnetic responsiveness, low cost, low energy consumption and no pollution, they can be detected on the surface of magnetic particles or magnetic Functional groups on the surface of particles (such as amino groups, hydroxyl groups, sulfhydryl groups, and ethylene oxide, etc.) immobilize biologically active substances such as enzymes, antibodies, and oligonucleotides. Traditional immunoassays mostly use enzyme-labeled plates as solid phase carriers. Suspended magnetic particles as a carrier have a higher specific area and can react more fully...

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Problems solved by technology

[0003] When performing detection, the magnetic particle luminescent immunoassay analyzer needs to use the cuvette to load and transport the analysis sample, and needs to go through the process of adding reagents, incubating, washing, adding substrate and mixing before the detection. The traditional magnetic particle luminescent immunoassay analyzer Generally, a disc-type single-channel circulation conveying structure is adopted. Generally, multiple cuvettes are fixed as a group for overall conveying or a single cuvette is conveyed one by one. When conveying in an integral manner, its flexibility is poor, and it is difficult to adapt to small batches of diverse samples. The need for detection, when a single cup is transported one by one, its transport efficiency is low, which seriously limits the detection efficiency of the magnetic particle luminescence immunoassay analyzer

[0004] In addition, the magnetic particle luminescence immunoassay is divided into one-step method and two-step method according to the number of reagent injections. When the two-step method is used, after the first reagent injection and incubation, the reagent needs to be added again and then added again. In the follow-up analysis process, when some test samples need to be detected by one-step method, and some need to be detected by two-step method, the traditional magnetic particle luminescence immunoassay analyzer can only be carried out one by one, which is difficult to meet its detection needs

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