Active hydrogel, preparation method and application thereof in operative wound healing

Abstract

The invention discloses an active hydrogel and a preparation method thereof. The method comprises the following steps: by taking rat groin adipose tissues without capillary and muscular tissues as raw materials, preparing an adipose tissue-derived stromal cell conditional medium; by taking chitosan and hyaluronic acid as raw materials, performing cross-linking reaction on chitosan and hyaluronic acid under the effect of a cross-linking agent glutaraldehyde, thereby preparing a chitosan-hyaluronic acid compound gel; and fully mixing the adipose tissue-derived stromal cell conditional medium and the chitosan-hyaluronic acid compound gel, adhering the active factors of the adipose tissue-derived stromal cell conditional medium to the chitosan-hyaluronic acid compound gel, adding I type collagen and uniformly mixing, thereby acquiring the active hydrogel. The active hydrogel provided by the invention can be used for repairing the skin and muscle injury in operative wound, the regeneration effect of the skin and muscle can be greatly promoted and the active hydrogel is convenient in a use process, high in safety, simple in preparation method and easy in industrialization.

Description

technical field [0001] The present invention relates to the technical field of damaged muscle and skin repair, more specifically, the present invention relates to a kind of active hydrogel, the present invention also relates to a kind of preparation method of active hydrogel, the present invention also relates to an active hydrogel Application of glue in surgical wound repair. Background technique [0002] Surgical trauma is the destruction of human tissues or organs after surgery. Surgical trauma can result in substantial loss of skeletal muscle and disruption of native tissue architecture. For muscle loss, current therapies mainly include surgical muscle flap transplantation, physical therapy and rehabilitation, but cannot effectively restore function. And the limitation and rejection of donor tissue affect the success rate and prognosis of muscle flap surgery. Infection, necrosis, and difficult-to-heal wounds are also complications easily caused by surgical trauma. Co...

AI Technical Summary

Problems solved by technology

Chitosan has a wide range of sources and is easy to prepare. It is the only hydrophilic poplar ion polymer in nature. It has good film-forming properties and hygroscopicity. It also has the characteristics of good biocompatibility, antibacterial properties and biodegradability. The hydrogel prepared with it has many unique features, but the chitosan gel is more brittle, weaker in viscoelasticity, insufficient in mechanical properties and weaker in water holding capacity

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