Method for preventing and controlling crop weeds

A technology for crops and weeds, applied in the field of weed control in the field, can solve the problems of limited types of herbicides, different use effects, and difficulty in achieving efficient and safe weeding, so as to reduce the cost of weed control, reduce the dosage, reduce the The effect of the risk of harm

Active Publication Date: 2017-10-31
HANGZHOU RUIFENG BIOTECH LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when applying this method to existing crops for post-emergence weed control, it is necessary to accurately grasp the amount of herbicide used and the conditions of use. It is very large, and the effect of use is also different, it is difficult to achieve the purpose of efficient and safe weeding

Method used

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  • Method for preventing and controlling crop weeds
  • Method for preventing and controlling crop weeds
  • Method for preventing and controlling crop weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Obtaining of herbicide-resistant transgenic plants

[0039] 1. Acquisition of plasmid vectors containing exogenous genes

[0040] Entrusted Shanghai Bioengineering Co., Ltd. to synthesize the first herbicide resistance gene-glyphosate gene G, its nucleotide sequence is SEQ ID NO.1, and the corresponding amino acid sequence is SEQ ID NO.2; entrusted Shanghai Bioengineering Co., Ltd. Three herbicide-resistant cytochrome oxidase genes X1, X2 and X3 containing the 35S terminator were synthesized, and the two ends of the gene were respectively provided with restriction sites of BamHI and KpnⅠ. The nucleotide sequence of X1 is SEQ ID NO.3 and the corresponding amino acid sequence is SEQ ID NO.4, the nucleotide sequence of X2 is SEQ ID NO.5 and the corresponding amino acid sequence is SEQ ID NO.6, and the nucleoside of X3 The acid sequence is SEQ ID NO.7 and the corresponding amino acid sequence is SEQ ID NO.8. The CMV35S promoter was amplified from the vector ...

Embodiment 2

[0061] Example 2: Weed control of herbicide-resistant transgenic corn

[0062] 1. Analysis of transgenic corn herbicide resistance

[0063] MGX1, MGX2, and MGX3 each select 5 transformed lines for herbicide resistance analysis, and spray the transgenic seedlings at the 4-5 leaf stage (1-8 spray once, and the rest spray 2 times, according to actual needs) Carry out multiple spraying) herbicides, herbicide application methods and usage rates are as follows:

[0064] 1) Glyphosate isopropylamine salt (40g / mu)+2,4-D (18g / mu), wherein glyphosate isopropylamine salt is 0.1wt%, 2,4-D600mg / L, and the solvent is water;

[0065] 2) Glyphosate isopropylamine salt (40g / mu) + nicosulfuron (2.4g / mu), wherein glyphosate isopropylamine salt 0.1wt%, nicosulfuron 80mg / L, solvent is water;

[0066] 3) Glyphosate isopropylamine salt (40g / mu) + mesotrione (5g / mu), wherein glyphosate isopropylamine salt 0.1wt%, mesotrione 100mg / L, solvent is water;

[0067] 4) Glyphosate isopropylamine salt (40g...

Embodiment 3

[0093] Example 3: Weed Control of Herbicide Resistant Transgenic Soybeans

[0094] 1. Analysis of genetically modified soybean herbicide resistance

[0095] SGX1, SGX2 and SGX3 respectively select 5 transformed lines to carry out herbicide resistance analysis, and spray herbicides (1-8 spraying once, the rest spraying 2 times, according to Actually need to carry out multiple spraying), the herbicide application method and usage amount are as follows:

[0096] 1) Glyphosate isopropylamine salt (40g / mu)+2,4-D (18g / mu), wherein glyphosate isopropylamine salt is 0.1wt%, 2,4-D600mg / L, and the solvent is water;

[0097] 2) Glyphosate isopropylamine salt (40g / mu) + nicosulfuron (1.2g / mu), wherein glyphosate isopropylamine salt 0.1wt%, nicosulfuron 80mg / L, solvent is water;

[0098] 3) Glyphosate isopropylamine salt (40g / mu) + mesotrione (2.5g / mu), wherein glyphosate isopropylamine salt 0.1wt%, mesotrione 100mg / L, solvent is water;

[0099] 4) Glyphosate isopropylamine salt (40g / mu...

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Abstract

The invention discloses a method for preventing and controlling crop weeds. The method comprises the following steps of spraying a first class of herbicide and / or a second class of herbicide in a 4-5-leaf stage of crops so as to prevent and control the weeds, wherein the spraying amount is 1g / mu-100g / mu, the first class of herbicide is glyphosate isopropamide, and the second class of herbicide is one or mixture of 2,4-D, dicamba, mesotrione, atrazine, bentazone, nicosulfuron, flazasulfuron or penoxsulam. By utilizing the method, the prevention and control efficiencies of the weeds can be improved, the risk of phytotoxicity caused by the herbicide to the crops can be reduced, and the production of resistant weeds can be effectively prevented. Compared with a weed prevention and control method of a traditional transgenic herbicide-resistant plant, the method has the advantages that the dosage of the herbicide can be effectively reduced, the prevention and control cost of the weeds of transgenic plant is lowered, and the pollution caused by the herbicide to an agroecological environment is reduced.

Description

(1) Technical field [0001] The present invention relates to the field of weed control, and more specifically, the present invention relates to the field weed control of broad-spectrum herbicide-resistant transgenic crops by using multiple herbicides. (2) Background technology [0002] Weed control is the main work in the crop cultivation process, and the use of chemical herbicides to control weeds is the most economical and effective method of weed control. Selective herbicides are widely used for post-emergence weeding of crops. When the application conditions and dosage range are strictly controlled, selective herbicides can effectively control weeds without harming crops. Selective herbicides have relatively strict safety conditions for applicable crops. In production and application, problems such as inaccurate herbicide dosage and loose control of application conditions often cause selective herbicides to affect applicable crops. Phytotoxicity, thereby affecting the gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/20A01N39/04A01N47/36A01N41/10A01N37/40A01N43/88A01N43/90A01P13/00
CPCA01N37/40A01N39/04A01N41/10A01N43/88A01N43/90A01N47/36A01N57/20A01N2300/00
Inventor 沈志成林朝阳张先文
Owner HANGZHOU RUIFENG BIOTECH LIMITED
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