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Bacillus amyloliquefaciens and application thereof to prevention and treatment of forest leaf spot disease

A technology for dissolving amyloid spores and bacilli, applied in the field of microorganisms, can solve problems such as non-ideal biocontrol agents, achieve good development and application prospects, facilitate production and application, and reduce use.

Inactive Publication Date: 2017-11-10
句容市林业科技推广中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no ideal biocontrol agent for the prevention and treatment of beech and ginkgo leaf spot. If biological pesticides can be used or the use of pesticides as little as possible can be used to effectively control beech and ginkgo leaf spot, pesticide residues will be reduced to a certain extent , greatly improving its medicinal safety

Method used

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  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of forest leaf spot disease
  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of forest leaf spot disease
  • Bacillus amyloliquefaciens and application thereof to prevention and treatment of forest leaf spot disease

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Experimental program
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Effect test

Embodiment 1

[0024] A strain of Bacillus amyloliquefaciens JRNW0001-1 was isolated from susceptible plants in various forest farms in Jurong City, Jiangsu Province. The strain was cultured on the PAD plate. Bacteria-positive bacteria, white, translucent, rod-shaped, colony diameter 3-5mm, round, with neat edges, smooth and moist, slightly raised. The bacterial strain was sent to the General Microorganism Center (CGMCC for short) of the China Microbiological Culture Collection Management Committee (abbreviated as CGMCC) located in Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, for preservation. The preservation number is CGMCC No.13749, and the preservation date is March 10, 2017. After identification, the strain was Bacillus amyloliquefaciens, named JRNW0001-1.

Embodiment 2

[0026] Microbial agents produced by Bacillus amyloliquefaciens JRNW0001-1: 4g of beef extract, 8 parts of peptone and 4.5g of sodium chloride were dissolved in 1000ml of distilled water, adjusted to a pH of 6.0, stirred evenly, and sterilized to obtain a fermentation medium. Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JRNW0001-1 is inoculated in the fermentation medium, fermented and cultured at 28-30° C. for 40-48 hours, and the fermented liquid obtained is used as a microbial agent.

Embodiment 3

[0028] Analysis of the effect of microbial agents produced by Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JRNW0001-1 on the control of beech and ginkgo leaf spot.

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof to prevention and treatment of forest leaf spot disease. The strain is JRNW0001-1 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.13749, and the preservation date is March 10, 2017. The bacillus amyloliquefaciens has remarkable effect of preventing and treating leaf spot disease of forest, particularly gingko trees and zelkova serrata and reduces the use of chemical pesticide; the microbial agent produced by the strain JRNW0001-1 can obviously prevent and treat the leaf spot disease of the gingko trees and the zelkova serrata, is simple in cultivation condition, easy in storage and easy in industrialized production, and has good development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of bacillus amyloliquefaciens and its application in preventing and treating forest leaf spot disease. Background technique [0002] Plant disease is one of the main factors affecting forestry production. However, at present, the prevention and control of plant diseases is mainly the use of chemical agents. After long-term use of chemical agents, they will bring residues, pollution, and resistance of harmful organisms to the earth. A series of serious problems such as target organisms and even destruction of ecological balance have prompted people to explore a new plant pest control strategy that is harmless to humans and the environment and has good control effects. With the general concern of the public on the safety of chemical pesticides in food and environment, the full use of beneficial microorganisms to control plant diseases has been paid more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 张红岩陈州王丹
Owner 句容市林业科技推广中心
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