Method for treating complementary determining regions 3 (CDRs3) of T cell antigen receptor beta chains

Abstract

The invention relates to a method for treating complementary determining regions 3 (CDRs3) of T cell antigen receptor beta chains. The method comprises the following steps: setting an experimental group and a control group, wherein each group comprises a plurality of parts of peripheral blood samples which are dissimilar to a human body and get away from the human body; separating T cells of the peripheral blood samples, and respectively extracting deoxyribonucleic acid (DNA) in the T cells of the peripheral blood samples; adopting a multiplex polymerase chain reaction (PCR) technique to amplify the CDRs3 of the T cell antigen receptor beta chains; carrying out high-throughput sequencing on the CDRs3 of the T cell antigen receptor beta chains, and carrying out DNA analysis on the T cell antigen receptor beta chains in the experimental group and the control group. The method for treating the CDRs3 of the T cell antigen receptor beta chains is reasonable and feasible in design, and can be used for effectively acquiring information, taken as intermediate results, about renal transplant recipients at the perioperative period and further verifying, analyzing and researching the information so as to help to study the pathogenesis of a rejection reaction and guide the individual rational application of immunosuppressive drugs, thus being beneficial to increment of short-term and long-term survival rate of renal transplant recipients after transplantation.

Description

technical field [0001] The invention relates to a method for acquiring information of kidney transplantation recipients in the perioperative period as an intermediate result, in particular to a processing method for T cell antigen receptor beta chain CDR3. Background technique [0002] Kidney transplantation is currently the most effective treatment for chronic renal failure. With the increasing number of patients with chronic renal failure, researchers have made continuous exploration and finally made kidney transplantation the best alternative treatment method for patients with end-stage renal disease. Successful kidney transplantation can significantly improve the quality of life of patients with end-stage renal disease. In recent years, the short-term survival rate of kidney transplant recipients / kidney transplantation has been significantly improved, but the long-term survival rate of kidney transplant recipients / kidney transplantation has not been further improved. S...

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Problems solved by technology

However, the sensitivity and specificity of serum creatinine are poor, which is of little significance for clinical prevention and treatment; color Doppler ultrasound is greatly affected by the subjective effects of ultrasound diagnosis doctors; it is more common to monitor the blood concentration of immunosuppressants, However, due to individual differences in drug metabolism in different recipients, and various factors in the body can affect the blood concentration of immunosuppressive drugs, many postoperative patients still inevitably have rejection; The examination is an invasive examination, and it can only reflect the situation of the punctured part of the transplanted kidney. It has certain risks for the patient, and there are complications such as bleeding, shock, arteriovenous fistula, hematuria, anuria, rupture of the transplanted kidney, and even It may cause the loss of transplanted kidney, etc., so it is not suitable to monitor repeatedly

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