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Stem cell composition and facial rejuvenation cosmetic preparation

A composition and stem cell technology, applied in cosmetic preparations, preparations for skin care, cosmetics, etc., can solve problems such as limited repair effect, and achieve the effects of improving skin texture, beauty and health skin physiological functions, and increasing skin thickness.

Inactive Publication Date: 2017-11-24
GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problem of limited repairing effect in the existing cosmetic technology, the present invention provides a new type of stem cell composition and facial rejuvenation cosmetic preparation, which has anti-wrinkle, whitening and moisturizing effects, good repairing effect and long maintenance time

Method used

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  • Stem cell composition and facial rejuvenation cosmetic preparation
  • Stem cell composition and facial rejuvenation cosmetic preparation
  • Stem cell composition and facial rejuvenation cosmetic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and culture of umbilical cord mesenchymal stem cells

[0021] 1. Take the umbilical cord of a healthy full-term caesarean section baby, perform aseptic cleaning in an ultra-clean bench, peel off the umbilical cord amniotic membrane and arteriovenous, and take out Wharton’s gum; cut it into pieces with surgical scissors, and add high-sugar DMEM+10% FBS complete medium Resuspended, then inoculated into 15cm dishes, transferred to 5% CO 2 , Cultivated in an environment of 37°C. Replenish the liquid every 5 days until the cells climb out, discard the tissue pieces, supplement new complete medium and continue the culture, and subculture after the cells form larger clones.

[0022] 2. Take subcultured P0 cells, discard the original culture medium in the dish, add 10mL PBS buffer solution to gently wash the cell growth surface, and discard the washing solution. Then, add 2 mL of 0.25% trypsin, shake the plate left and right so that the trypsin evenly cove...

Embodiment 2

[0026] Prepare A, B and C three kinds of facial rejuvenation beauty preparations according to the formula described in Table 1 respectively, and its specific preparation process is as follows:

[0027] 1. Preparation of pentapeptide mother liquor: Weigh pentapeptide powder, add saline, dissolve to obtain pentapeptide mother liquor with a concentration of 500 mg / mL, filter through a 0.22 μm membrane filter, and store in a 4°C refrigerator for later use.

[0028] 2. Preparation of EGF solution: Take out a bottle of EGF freeze-dried powder and a bottle of solvent respectively, absorb the solvent with a disposable syringe, pour it into the bottle of EGF freeze-dried powder, and mix well to form 2.5mL of EGF solution.

[0029] 3. Add hyaluronic acid to the EGF solution to obtain liquid A; resuspend the umbilical cord mesenchymal stem cells prepared in Example 1 with physiological saline to obtain the umbilical cord mesenchymal stem cell suspension, and then add pentapeptide prepared...

Embodiment 3

[0032] Example 3 Evaluation of facial anti-wrinkle effect

[0033] Select 120 healthy adults (half male and half female) with obvious wrinkles on the face, aged 40 to 60 years old; randomly divided into 4 groups, including 3 groups of control groups and 1 group of experimental groups, and then the preparations shown in Table 2 Inject into the deep dermis where there are many facial wrinkles respectively, and evaluate the anti-wrinkle effect after 4 weeks. The evaluation indicators are: the cosmetic effect is scored according to the degree of facial wrinkle repair, the better the repair, the higher the score; the maintenance time is the time span from the appearance of the cosmetic effect to the loss of the cosmetic effect. As shown in the results in Table 3, the wrinkle repair effect and maintenance time of Example 1 are much higher than those of the control groups 1 to 3, showing its good cosmetic effect.

[0034] Table 2

[0035] group

Component

Contro...

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Abstract

The invention belongs to the field of cosmetics, and particularly relates to a stem cell composition and a facial rejuvenation cosmetic preparation. The stem cell composition comprises umbilical cord mesenchymal stem cells, hyaluronic acid, pentapeptide and EGF, the compatibility of medicines is reasonable, the synergistic effect is achieved, so that the stem cell composition simultaneously has the functions of wrinkle preventing, skin whitening and moisturizing, and the maintenance time is longer. The stem cell composition is applied to preparation of the facial rejuvenation cosmetic preparation, facial wrinkles can be eliminated, the skin texture is improved, the complexion is brightened, the repairing effect is good, and the maintenance time is long.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a stem cell composition and a cosmetic preparation for facial rejuvenation. Background technique [0002] Face rejuvenation refers to various means and methods that can restore a youthful appearance to the aging face. Facial aging is the result of common changes in the soft and hard tissues of the body and the face / neck. The main manifestations are: thin and dry facial skin, weakened skin elasticity, skin pigmentation, dull and uneven skin tone, increased wrinkles, and reduced subcutaneous fat tissue. Local accumulation, sagging caused by loose supporting structures, muscle atrophy. Facial aging is closely related to factors such as physical fitness, genetic factors, living environment, psychological emotions, working environment, nutritional status, disease, and local skin care. The average person begins to show signs of aging after the age of 25, and the formation of faci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/64A61K8/73A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/99A61K8/64A61K8/735A61K2800/91A61Q19/00A61Q19/02A61Q19/08
Inventor 马颖马铎阮雯莉
Owner GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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