Method for green extraction and determination of multiple compounds in Chinese herbal compound drug Naoxintong

The technology of a compound, Naoxintong, is applied in the field of green extraction and determination of various compounds in the traditional Chinese medicine compound Naoxintong, which can solve the problems of large amount of extraction reagents and long time, so as to reduce the potential harm to the environment and reduce the amount of use , has the effect of application prospect

Active Publication Date: 2017-12-01
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

However, the traditional MSPD sample dosage is 0.5-1g, the adsorbent dosage is 1-4g, the elution volume is 5-20mL, the extraction reagent dosage is large, and the time is long

Method used

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  • Method for green extraction and determination of multiple compounds in Chinese herbal compound drug Naoxintong
  • Method for green extraction and determination of multiple compounds in Chinese herbal compound drug Naoxintong
  • Method for green extraction and determination of multiple compounds in Chinese herbal compound drug Naoxintong

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preparation example Construction

[0070] 1.5 The preparation method of the extract of Naoxintong sample

[0071] Accurately weigh 25mg of Naoxintong capsule powder and a certain amount of adsorbent and mix them evenly in a mortar. After fully grinding, transfer the mixture to a 1mL SPE tube with a sieve plate at the bottom, and gently press it with another sieve plate. Then put it on the extraction device connected with a vacuum pump, elute with eluent to obtain the eluate, centrifuge the eluate twice for 10min under the condition of 14,000rpm, and take the supernatant as Naoxintong Extract of the sample; this extract was used for UPLC analysis.

[0072] 2 Determination of matrix solid phase dispersion extraction parameters

[0073] 2.1 Selection of adsorbent

[0074] Here we mainly investigate the influence of different adsorbents on the extraction effect of 16 compounds, Alumina-A (acidic alumina), Alumina-B (basic alumina), Alumina-N (neutral alumina), C18 (18 Alkyl bonded silica gel), Celite AZO (diatom...

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Abstract

Embodiments of the invention provide a method for green extraction and determination of a variety of compounds in the Chinese herbal compound drug Naoxintong. The method comprises the following steps: (1) acquiring liquid extract of a Naoxintong sample by using a matrix solid-phase dispersion extraction process, wherein the mass of the Naoxintong sample is 10-50 mg, an adsorbent is magnesium silicate, a mass ratio of the Naoxintong sample and the adsorbent is (2: 1) to (1: 5), an eluent is methanol with a concentration of 60% to 100 % and contains 0-0.2% by volume of formic acid, the ratio of the mass of the Naoxintong sample to an elution volume is 25-42 mg/mL, and grinding time of the Naoxintong sample and the adsorbent is 0-8 minutes; and (2) analyzing the liquid extract of the Naoxintong sample by using ultra-high performance liquid chromatography so as to determine the contents of the compounds in the Chinese herbal compound drug Naoxintong. The determination method provided by the invention can provide a theoretical basis for the validation studies of traditional Chinese medicines and provide technical support for the quality control of Naoxintong.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine quality control, in particular to a green extraction and determination method for various compounds in the traditional Chinese medicine compound Naoxintong. Background technique [0002] Naoxintong is a traditional Chinese medicine compound preparation composed of 16 traditional Chinese medicines, including 11 herbal medicines: astragalus, red peony root, salvia miltiorrhiza, angelica, chuanxiong, peach kernel, safflower, milletus, achyranthes bidentata, cassia twig, and mulberry branch; 2 kinds of resinous traditional Chinese medicine: frankincense (made), myrrh (made); and 3 kinds of animal medicine: earthworm, scorpion, and leech. According to the ancient book "Yilin Cuo Gai" of the Qing Dynasty, Naoxintong originated from Buyang Huanwu Decoction, which has the effects of nourishing qi, activating blood, removing blood stasis and dredging collaterals. , Numbness of the lim...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 常艳旭王慧琳李晋何俊王晖王虹陈璐
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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