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Method for increasing hybrid setting rate of carrots

A technology of radish hybridization and seed setting rate, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of incompatibility reaction, low hybrid seed setting rate at flowering stage, and low radish hybrid seed setting rate, so as to increase yield , good operability and application value, and the effect of reducing the cost of seed production

Active Publication Date: 2017-12-15
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

However, in the actual breeding process, when the first-generation hybrids are prepared from two superior materials, sometimes the hybrid seed setting rate at the flowering stage is low. The main reason is that the two radish materials have the same S haplotype, which leads to incompatibility reactions. happened
Therefore, how to solve the problem of low seed setting rate of radish hybrids caused by self-incompatibility has become very urgent

Method used

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  • Method for increasing hybrid setting rate of carrots
  • Method for increasing hybrid setting rate of carrots
  • Method for increasing hybrid setting rate of carrots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Identification of the parental S haplotype of "Jinghong 3"

[0026] "Jinghong No. 3" is one of the new red-skinned radish varieties bred by the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences. It has the advantages of bright red fleshy roots, white flesh, round roots, strong disease resistance, and good cooked food quality. , Suitable for autumn planting in North China and Northeast China. Only about 20% of the female parent plants of this variety use the male sterile line to produce seeds with normal fruiting per plant (compatible per plant), and the yield per mu is about 20 kg ( figure 1 ), the high cost of seed production restricts its further promotion and application. The analysis suggests that it may be due to the fact that the parents have the same S haplotype, which caused the hybrid incompatibility and the low seed setting rate. Therefore, it is necessary to identify the S haplotype of the parents.

[0027] (1) The test ...

Embodiment 2

[0033] Example 2. Development and auxiliary selection of S-11 and S-38 haplotype polymorphism markers

[0034] Such as Figure 4 As shown, the DNAMAN software comparison found that the above-mentioned S-11 and S-38 haplotype SRK genes have 9 base differences, and they contain polymorphic Taq a I endonuclease cleavage site (5'-AGCT-3'). The PCR-RFLP marker was developed based on the variation of this locus for screening of hybrid compatible single plants of "Jinghong 3".

[0035] 300 strains of male parent, sterile line (maternal parent) and its maintainer line of "Jinghong 3" were selected respectively, and the above-mentioned PCR-RFLP markers were used for affinity screening. Such as figure 2 As shown, the S haplotypes of the male parent are all S-11, the S haplotype S-38 in the female parent sterile line accounts for 15.5%, and the S haplotype S-11 accounts for 84.5%. The individual plants with S haplotypes S-38 and S-11 in the line account for 2% and 98%, respectively.

[003...

Embodiment 3

[0037] Example 3. Production test

[0038] In December 2015, molecular markers were used to select female parent sterile lines of "Jinghong 3", and the female parent plants with S haplotypes of S-38, S-11 and S-11 / S-38 were selected. Twenty plants were planted in the same seed production shed as the male parent (S haplotype S11), and 2 bees were placed at the initial flowering stage for hybrid seed production experiment. Such as image 3 As shown, the number of pods of the female parent material with the S-38 haplotype was significantly more than that of the female parent material with the S haplotype S-11, and 40.3 g and 450.2 g of seeds were harvested respectively. This indicated that the use of markers to select the S haplotype of the parents of "Jinghong 3" could significantly increase the yield of hybrid seed production.

[0039] In the autumn of 2016, hybrids of "Jinghong 3" harvested from female parents with S haplotypes of S-38 and S-11 were sown in open fields, and the au...

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Abstract

The invention discloses a method for increasing the hybrid setting rate of carrots. According to the method, the problem that the hybrid seed setting rates of two carrot parent materials with excellent comprehensive characters and good combining abilities are low in a hybrid seed production process is solved; by utilizing a molecular marker technique, the biparental affinity is improved, so that the seed production yield is increased, and the seed reproduction cost is lowered. The method comprises the steps of firstly authenticating S unit forms of a biparental self-incompatibility gene, developing molecular markers among the different S unit forms, carrying out marker-assisted selection on a biparental material, screening to obtain single plants with inconsistent S unit forms between the parents, carrying out parent propagation, and applying propagated parents to the hybrid seed production. According to the method, the problem of low hybrid setting rate of the carrots caused due to the biparental incompatibility can be solved, and the seed production yield can be remarkably increased.

Description

Technical field [0001] The invention relates to a method for improving the hybridization rate of radish, and belongs to the technical field of molecular plant breeding. Background technique [0002] Radish is an important cruciferous vegetable crop and is widely cultivated all over the world. Radish is a cross-pollinated crop with obvious heterosis. Self-incompatible lines and male sterile lines are important ways for radish hybrid seed production and utilization of heterosis. However, in the actual breeding process, the two excellent materials sometimes have a low seed setting rate during the flowering period during the preparation of the first hybrid. The main reason is that the two radish materials have the same S haplotype, which leads to incompatibility. happened. Therefore, how to solve the problem of low hybridization rate of radish caused by self-incompatibility has become very urgent. [0003] Self-incompatibility (SI) of plants is widespread in higher plants and is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 张丽王庆彪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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