Micro-injection method of Bactrocera dorsalis eggs
A technology of B. citrifolia and microinjection, which is applied in the field of microinjection of B. citrifolia eggs, can solve problems such as excessive internal pressure and difficult drying of B. citrifolia eggs, and achieves easy collection and guaranteed freshness. and synchronization, the effect of convenient micromanipulation
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[0030] The collection method of Bactrocera dorsalis ovum described in the present embodiment comprises the following steps:
[0031] A centrifuge tube perforated on the tube wall, the centrifuge tube can have a volume of 25-100ml according to the amount of eggs collected, and the present embodiment adopts a 50ml centrifuge tube, and adds 10ml of orange juice (the fruit orange from Minute Maid, and filters out the fruit) ), constantly shaking to make orange juice fully contact the centrifuge tube inner wall and then putting it into the Bactrocera dorsalis rearing cage capable of laying eggs, the placement time is 20-30min, Bactrocera dorsalis lays eggs in the centrifuge tube, and obtains the oviposition tube;
[0032] Take out the oviposition tube, rinse with clear water and collect the ovum on the centrifuge tube inner wall on the black gauze, promptly collect the Bactrocera dorsalis ovum; Carry out the microinjection method of the Bactrocera dorsalis ovum according to the foll...
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