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Streptococcus thermophilus enrichment medium and preparation method thereof

An enrichment medium and a technology for Streptococcus thermophilus, which are applied in the field of Streptococcus thermophilus enrichment medium and its preparation, can solve the problems of difficult operation, high price of the medium, slow proliferation rate, etc. Growth rate, speed-up effect

Inactive Publication Date: 2017-12-19
ANYANG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MRS medium has a wide range of applications and can be used to cultivate almost all lactic acid bacteria, but the proliferation speed is slow and the price is high
Whey protein medium is easy to obtain and widely used, but the survival rate of bacteria cultured with this medium is low due to the denaturation and precipitation of whey protein
Skim milk medium is as simple and easy to obtain as whey protein medium, and the price is low, but the medium has a high viscosity and is difficult to operate
Skim milk enzymatic hydrolysis medium is a good basic medium for the reproduction of Streptococcus thermophilus. By using enzymes to decompose macromolecular nutrients, the macromolecular substances that are not easily absorbed and utilized are converted into small molecules that are easily absorbed and utilized. Molecular substances are beneficial to the proliferation of bacteria, but the price of the medium is high, which is not conducive to industrial production. The medium used in industrial production generally requires simple and easy ingredients, easy operation, high survival rate of bacteria, and fast proliferation , and the culture medium and bacteria are easy to separate

Method used

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  • Streptococcus thermophilus enrichment medium and preparation method thereof
  • Streptococcus thermophilus enrichment medium and preparation method thereof
  • Streptococcus thermophilus enrichment medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A streptococcus thermophilus enrichment culture medium, every 100mL enrichment medium comprises the corn boil liquid that is made from 3-5g corn and the tomato boil liquid that 3-5g tomato makes, also includes 2-4g brown sugar, The balance is water.

[0043]Every 100mL enrichment culture medium comprises the corn boiling liquid that is made from 4g corn and the tomato boiling liquid that 4g tomato is made, also comprises 3g brown sugar, and balance is water.

[0044] The preparation method of the corn cooking liquid is as follows: the corn is washed, peeled, soaked in water for 10-15 hours, taken out, boiled in water for 15-20 minutes, and filtered with eight layers of gauze to obtain the supernatant.

[0045] The preparation method of the tomato cooking liquid is as follows: the tomatoes are washed, peeled, soaked in water for 10-15 hours, taken out, boiled in water for 15-20 minutes, and filtered with eight layers of gauze to obtain the supernatant.

[0046] As the p...

Embodiment 2

[0051] A Streptococcus thermophilus enrichment culture medium, every 100mL enrichment culture medium comprises the corn boil liquid that is made from 3g corn and the tomato boil liquid that 3g tomato makes, also includes 2g brown sugar, and balance is water.

[0052] As the preparation method of the above-mentioned Streptococcus thermophilus enrichment medium, it is characterized in that: the specific steps are as follows:

[0053] (1) Wash and peel the corn, soak it in water for 10 hours, remove it, boil it in water for 15 minutes, filter it with eight layers of gauze to get the supernatant, and obtain the corn cooking solution;

[0054] (2) Wash and peel the tomatoes, soak them in water for 10 hours, take them out, boil them in water for 15 minutes, filter them with eight layers of gauze to get the supernatant, and get the tomato cooking solution;

[0055] (3) Mix corn cooking liquid and tomato cooking liquid, add brown sugar, dissolve and adjust the pH to 6.

[0056] The p...

Embodiment 3

[0058] A kind of Streptococcus thermophilus enrichment culture medium, every 100mL enrichment culture medium comprises the corn boil liquid that is made from 3.5g corn and the tomato boil liquid that 3.5g tomato makes, also includes 2.5g brown sugar, and the balance is water.

[0059] As the preparation method of the above-mentioned Streptococcus thermophilus enrichment medium, it is characterized in that: the specific steps are as follows:

[0060] (1) Wash and peel the corn, soak it in water for 11 hours, remove it, boil it in water for 16 minutes, filter it with eight layers of gauze to get the supernatant, and obtain the corn cooking solution;

[0061] (2) Wash and peel the tomatoes, soak them in water for 11 hours, take them out, boil them in water for 16 minutes, filter them with eight layers of gauze to get the supernatant, and get the tomato cooking solution;

[0062] (3) Mix corn cooking liquid and tomato cooking liquid, add brown sugar, dissolve and adjust the pH to...

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Abstract

The invention discloses a streptococcus thermophilus enrichment medium. The enrichment medium of every 100mL comprises corn cooking liquid obtained by 3-5g of corn and tomato cooking liquid obtained by 3-5g of tomatoes and also comprises 2-4g of brown sugar and the balance of water. The streptococcus thermophilus enrichment medium takes streptococcus thermophilus as a research object, and common food materials in life as main raw materials, and proliferation and optimization of a streptococcus thermophilus culture medium are carried out. The raw materials are low in cost and easily available, and the streptococcus thermophilus proliferation rate is improved, so that the speed of mass production of streptococcus thermophilus is increased.

Description

technical field [0001] The invention belongs to the technical field of probiotic culture, and in particular relates to a streptococcus thermophilus enrichment medium and a preparation method thereof. Background technique [0002] There are many kinds of culture medium for Streptococcus thermophilus. At present, there are four most common ones: MRS medium, whey protein medium, skim milk medium, and skim milk enzymatic hydrolyzate medium. MRS medium has a wide range of applications and can be used to cultivate almost all lactic acid bacteria, but the proliferation rate is slow and the price is high. Whey protein medium is easy to obtain and is widely used, but the survival rate of bacteria cultured with this medium is low because whey protein is prone to denaturation and precipitation. Skim milk medium is as simple and easy to obtain as whey protein medium, and the price is low, but the medium has a high viscosity and is not easy to operate. Skim milk enzymatic hydrolysis me...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/46
CPCC12N1/20
Inventor 杜磊刘书梅谷令彪杜鹃杨利玲杜杨吴丽媛李聪聪杜雨童
Owner ANYANG INST OF TECH
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