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A rapid detection method for fresh milk

A detection method and technology for raw milk, applied in the field of pathogen detection, can solve the problems of long detection time, complicated technical operation and high cost, and achieve the effects of simple operation, low price and reduced detection cost.

Active Publication Date: 2020-03-20
INST OF ANIMAL HUSBANDRY & VETERINARY SCI WUHAN ACADEMY OF AGRI SCI
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Problems solved by technology

At present, the detection methods for the total number of microbial colonies in fresh milk include microbial methods, physical and chemical methods, immunological methods, PCR technology, gene chips, and flow cytometry, etc., but the detection of these technologies has been around for a long time, the cost is high, and the technical operation is complicated. and other problems, it is difficult to widely popularize and apply in the detection of fresh milk

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  • A rapid detection method for fresh milk
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Embodiment Construction

[0022] Below, the applicant will further describe the method of the present invention in detail in combination with specific embodiments and accompanying drawings.

[0023] 1. Isolation and identification of contaminating microorganisms in fresh milk.

[0024] Isolation of main pathogenic bacteria: The fresh milk comes from the first two handfuls of milk from the dairy farm, a total of 60 parts. Take raw milk samples and put them on nutrient agar solid medium, 10% fresh rabbit fiber blood nutrient agar medium, potato solid (PDA) medium, trypticase soybean broth (TSB) medium, LB liquid medium plate respectively Streak directly, culture at 37°C for 24-48 hours, carry out aerobic isolation culture, observe and isolate a single colony, record its characteristics, and continue to identify. At the same time, directly inoculate fresh milk samples in LB liquid medium, cover with liquid paraffin, incubate at 37°C for 24h, inoculate on nutrient agar solid medium, and incubate at 37°C f...

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Abstract

The invention belongs to the technical field of rapid detection methods for the main pathogenic bacteria in raw fresh milks, and specifically discloses a rapid detection method for whether a raw fresh milk is qualified. According to the present invention, a novel safe chromogen reagent TMB is used, and reacts with catalase produced by contamination bacteria in a raw fresh milk to form a soluble light blue end product, the reaction can be terminated with 2M H2SO4 (producing a yellow precipitate), and the OD value of the reaction is read at 450 nm to indirectly reflect the concentration of the catalase produced by the bacteria in the raw fresh milk, such that the bacterial contamination degree of the raw fresh milk can be determined. With the method of the present invention, the bacterial content in the raw fresh milk can be accurately, rapidly and conveniently detected within 15 min, multiple samples can be simultaneously determined, and the technical problem of the detection of the main pathogenic bacteria in the raw fresh milk can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of pathogenic bacteria detection, and relates to a rapid detection method for whether fresh milk is qualified or not. Background technique [0002] Dairy products are nutritional products suitable for all ages, and raw milk is the source and key to the quality of dairy products. In recent years, dairy safety incidents have occurred frequently. Most of them are large-scale dairy poisoning incidents caused by contamination of pathogenic bacteria, and these incidents have once again brought dairy safety issues into the focus of attention from all walks of life. [0003] In my country, due to unreasonable feeding management, raw milk production and circulation links are easily polluted, causing the total number of bacterial colonies to exceed the standard, which poses a potential threat to consumers' health and seriously affects consumers' physical and mental health. The content of microorganisms in raw milk i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28
CPCG01N1/28G01N21/3103
Inventor 何斌陈洁邵志勇姚艳丰杨文海陈夏冰刘武金尔光童伟文席凤琴陶弼菲者永辉
Owner INST OF ANIMAL HUSBANDRY & VETERINARY SCI WUHAN ACADEMY OF AGRI SCI
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