Primer for detecting sheep mycoplasma pneumoniae

Abstract

The invention provides a primer for detecting sheep mycoplasma pneumonia. The sequence of the primer comprises an upstream primer: 5'-GGGACTTCGGGACTTATTGGA-3', and a downstream primer: 5'-CACGAGATGCAAACTGATTTACTTG-3'. According to a p80 gene sequence of KM435069.1 logged in GenBank, a specific primer is designed, a real-time fluorescence quantification PCR (polymerase chain reaction) method is established for a p80 gene of sheep mycoplasma pneumonia for a first time, and the method is good in specificity, high in sensitivity and good in repeatability and can be applied to diagnosis and epidemiological investigation on sheep mycoplasma pneumonia.

Description

technical field [0001] The invention relates to a primer for detecting sheep mycoplasma pneumoniae, which belongs to the field of molecular biology. Background technique [0002] Mycoplasma ovis pneumoniae ( Mycoplasma ovipneumoniae , Mo) is the main pathogen causing Mycoplasma pneumonia of goats and sheep (MPGS). MPGS is highly contagious, mainly through respiratory tract infection, and can also be transmitted vertically. Sick sheep and resistant sheep are the main sources of infection of the disease. The clinical symptoms of MPGS are high fever, cough, panting, progressive emaciation, and serous and fibrinous inflammation of the lung and pleura. . At present, there are reports of the disease occurring in Africa, Spain, Italy, the United States, Jordan abroad, Sichuan, Guizhou, Inner Mongolia, Qinghai, Guangxi, Fujian and other places in China, causing serious economic losses to the sheep industry. [0003] The diagnostic methods for Mo at home and abroad mainly include...

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Problems solved by technology

[0003] The diagnostic methods for Mo at home and abroad mainly include the isolation and identification of mycoplasma, conventional PCR, serological methods, and SYBRGreen I real-time fluorescent quantitative PCR. PCR has low sensitivity and cannot be quantified; while serological methods have poor specificity and sensitivity, and are prone to cross-reaction with mycoplasma of the same genus; although there are domestic and foreign evidences such as Yang et al. p113 Gene, Mo 16S rRNA, tuf gene and NA gene were respectively established Mo SYBR Green I real-time fluorescent quantitative PCR, but no Mo adhesin gene p80 The real-time fluorescence quantitative PCR method, the present invention according to the Mo adhesin gene p80 Sequence-specific primers were established for the first time against Mo p80 Gene real-time fluorescence quantitative PCR method, in order to provide technical support for MPGS in early clinical diagnosis and prevention

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