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Extracellular metabolites of highly active Lactobacillus plantarum tomato juice medium and its preparation method and application

A technology of tomato juice medium and extracellular metabolites is applied in the application field of microbial metabolites, and can solve the problems of unsatisfactory antioxidant effect, reduced bacterial activity, and unsuitability for human consumption.

Active Publication Date: 2021-08-06
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, currently on the market, lactic acid bacteria (Lactobacillus plantarum) food (lactic acid bacteria culture medium) is added with different contents of inorganic salts and surfactants to promote its growth and avoid agglomeration and precipitation, and add preservatives to prolong the shelf life but reduce the bacteria. Activity
The effect of two strains of lactic acid bacteria on the antioxidant activity of mouse serum and liver tissue (Food Research and Development, October 2010, Volume 31, Issue 10, Huang Shanshan, etc.) found that the metabolites of Lactobacillus have an antioxidative effect on mouse serum and liver tissue However, it uses MRS medium, and its metabolites have high concentrations of inorganic salts and surfactants, which are not suitable for human consumption, and their metabolites are not ideal for antioxidant effects

Method used

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  • Extracellular metabolites of highly active Lactobacillus plantarum tomato juice medium and its preparation method and application
  • Extracellular metabolites of highly active Lactobacillus plantarum tomato juice medium and its preparation method and application
  • Extracellular metabolites of highly active Lactobacillus plantarum tomato juice medium and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0059] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomatoes, add 1000ml of water to conditioner to extract the juice, filter through 160 mesh to obtain fresh tomato juice, add 2% glucose, 1% soybean protein, and 0.5% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0060] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0061] 1) The number of bacteria obtained with tomato juice medium is 10 8 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 20 hours to obtain a Lactobacillus plantarum seed solution;

[0062] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 20 hours at an inoculum size of 1%, and place it in a refrigerat...

Embodiment 2

[0065] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomato, add 1000ml of water to extract the juice from a conditioner, filter through 160 mesh to obtain fresh tomato juice, add 1.5% glucose, 1.5% soybean protein, and 0.7% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0066] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0067] 1) The number of bacteria obtained with tomato juice medium is 10 7 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 18 hours to obtain a Lactobacillus plantarum seed liquid;

[0068] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 18h at an inoculum size of 1%, place it in a refrigerator at...

Embodiment 3

[0070] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomato, add 1000ml of water to conditioner to extract the juice, filter through 160 mesh to obtain fresh tomato juice, add 2.5% glucose, 0.5% soybean protein, and 0.3% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0071] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0072] 1) The number of bacteria obtained with tomato juice medium is 10 9 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 24 hours to obtain a Lactobacillus plantarum seed liquid;

[0073] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 16h at an inoculum size of 1%, put it in a refrigerator at 6°C f...

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Abstract

The invention relates to an extracellular metabolite of a high-activity Lactobacillus plantarum tomato juice medium, a preparation method and an application thereof, and belongs to the technical field of application of microbial metabolites. The medium formula used in the preparation of the extracellular metabolite of plantarum lactobacillus is 1.5-2.5% glucose, 0.5-1.5% soybean protein, 0.3-0.7% yeast extract, and the balance is tomato juice. The formula is simple without adding buffer system inorganic salts and surfactants, and the metabolites obtained are suitable for human consumption. In the present invention, the extracellular metabolites of the Lactobacillus plantarum tomato juice medium have a protective effect on cell oxidative stress damage through the double verification of isolated cells and live biological experiments, and can improve the immune defense function of the body; and through the induction of acute alcoholic liver injury Experiments have found that the extracellular metabolites of the tomato juice medium of the present application have a good protective effect on animal liver damage.

Description

technical field [0001] The invention relates to an extracellular metabolite of a high-activity Lactobacillus plantarum tomato juice medium, a preparation method and an application thereof, and belongs to the technical field of application of microbial metabolites. Background technique [0002] During the normal physiological activities in the body, there are oxidation reactions involving the participation of oxidative active substances. The free radical scavenging system in the human body can maintain the dynamic balance of oxygen free radicals. The dynamic balance of free radical metabolism is the basic element to maintain life and health. When the new free radicals produced exceed their own antioxidant capacity, or the excessive intake of exogenous oxidative substances will lead to excessive accumulation of oxygen free radicals in cells Oxidative stress caused by excessive free radicals is one of the important reasons for the damage and disease of various tissues and organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A61K36/81A61P1/00A61P1/16A61P39/00C12R1/25A61K35/747
Inventor 曾爱兵
Owner WENZHOU MEDICAL UNIV
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